1.16.1.1: mercury(II) reductase

This is an abbreviated version!
For detailed information about mercury(II) reductase, go to the full flat file.

Word Map on EC 1.16.1.1

1.16.1.1

organomercurial

mercury-resistant

hgcl2

methylmercury

lipoamide

phytoremediation

mercury-contaminated

ferrooxidans

hg-resistant

geothermal

metal-resistant

phenylmercury

mercury-polluted

environmental protection

Reaction

Synonyms

bacterial mercuric reductase, Mer A, MerA, MerA protein, mercurate(II) reductase, mercuric (II) reductase, mercuric ion reductase, mercuric reductase, mercury reductase, Msed_1241, MseMerA, reduced NADP:mercuric ion oxidoreductase, reductase, mercurate(II), Rm CH34, Tn501 MerA, Tn501 mercuric ion reductase

ECTree

1 Oxidoreductases

1.16 Oxidizing metal ions

1.16.1 With NAD⁺ or NADP⁺ as acceptor

1.16.1.1 mercury(II) reductase

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Results	in table
20	Activating Compound
6363	AA Sequence
8	Application
1	CAS Registry Number
53	Cloned(Commentary)
197	Cofactor
4	Crystallization (Commentary)
7	Expression
25	General Information
3	General Stability
1	IC50 Value
59	Inhibitors
1	kcat/KM [mM/s]
3	Ki Value [mM]
33	KM Value [mM]
2	Localization
5	Metals/Ions
26	Molecular Weight [Da]
74	Natural Substrates/ Products (Substrates)
209	Organism
1	Oxidation Stability
3	Pathway
12	PDB ID
17	pH Optimum
4	pH Range
2	pI Value
33	Protein Variants
18	Purification (Commentary)
5	Reaction
1	Reaction Type
172	Reference
14	Source Tissue
10	Specific Activity [micromol/min/mg]
1	Storage Stability
217	Substrates and Products (Substrate)
43	Subunits
140	Synonyms
1	Systematic Name
19	Temperature Optimum [°C]
3	Temperature Range [°C]
10	Temperature Stability [°C]
15	Turnover Number [1/s]

Purification

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Purification on EC 1.16.1.1 - mercury(II) reductase

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8 entries

purification of the major 14C-labeled peptide from a tryptic digestion of labeled mercuric reductase

Pseudomonas aeruginosa

438077

recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis,

Lysinibacillus sphaericus

recombinant enzyme

recombinant His-tagged enzyme by nickel affinity chromatography from Escherichia coli strain BL21(DE3)

Metallosphaera sedula

A4YG49

744965

recombinant His-tagged MerA from Escherichia coli strain BL21(DE3)Plys by nickel affinity chromatography

plasmid R100

713700

recombinant MerA catalytic core and NmerA proteins from Escherichia coli strain XL-1 Blue by anion exchange chromatography and gel filtreation, and separation by affinity chromatography

Serratia marcescens

E0XF09

714219

recombinant MerA from Escherichia coli strain CM037

Cupriavidus metallidurans

744823

recombinant wild-type and mutant N-terminally His6-tagged and maltose-binding protein fusion enzymes from Escherichia coli strain C43 by amylose affinity chromatography, cleavage of the tags by 3C protease, ultrafiltration, and gel filtration

Shigella flexneri

728149