1.17.1.1: CDP-4-dehydro-6-deoxyglucose reductase

This is an abbreviated version!
For detailed information about CDP-4-dehydro-6-deoxyglucose reductase, go to the full flat file.

Reaction

Synonyms

ascD, CDP-4-keto-6-deoxy-D-glucose-3-dehydrogenase system, CDP-4-keto-deoxy-glucose reductase, CDP-6-deoxy-D-xylo-4-hexulose-3-dehydrase, cytidine diphosphate 4-keto-6-deoxy-D-glucose-3-dehydrogenase, More, NAD(P)H:CDP-4-keto-6-deoxy-D-glucose oxidoreductase, reductase, cytidine diphospho-4-keto-6-deoxy-D-glucose, rfbH

ECTree

1 Oxidoreductases

1.17 Acting on CH or CH₂ groups

1.17.1 With NAD⁺ or NADP⁺ as acceptor

1.17.1.1 CDP-4-dehydro-6-deoxyglucose reductase

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Results	in table
166	AA Sequence
5	Application
1	CAS Registry Number
3	Cloned(Commentary)
17	Cofactor
35	Inhibitors
1	KM Value [mM]
2	Metals/Ions
9	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
13	Organism
4	Pathway
2	pH Optimum
1	pH Range
1	Protein Variants
5	Purification (Commentary)
15	Reaction
3	Reaction Type
11	Reference
5	Specific Activity [micromol/min/mg]
5	Storage Stability
14	Substrates and Products (Substrate)
8	Subunits
17	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Purification

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Purification on EC 1.17.1.1 - CDP-4-dehydro-6-deoxyglucose reductase

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Salmonella enterica subsp. enterica serovar Typhimurium

437993

enzyme E1: using filtration on a Sephadex G-100 column and column chromatography on DEAE-cellulose, enzyme E3: using two filtrations on Sephadex G-100 columns

Yersinia pseudotuberculosis

437993

of cofactor, using streptomycin sulfate precipitation, ammonium sulfate precipitation and dialysis, DEAE-cellulose chromatography, ultrafiltration, Bio Gel P-2 filtration and ion exchange chromatography on Dowex-1

Yersinia pseudotuberculosis

437992

the first three steps are common to the purification of enzyme E1, enzyme E3 and cofactor, their separation can be accomplished after step 4, step 1: preparation of the crude extract, step 2: streptomycin sulfate precipitation, step 3: ammonium sulfate precipitation and dialysis, step 4: DEAE-cellulose chromatography, step 5: purification of enzyme E1, gel filtration on Sephadex G-100, step 6: second DEAE-cellulose chromatography, step 7: preparative polyacrylamide gel electrophoresis, step 5': purification of enzyme E3, gel filtration on Sephadex G-100, step 6': second DEAE-cellulose chromatography, step 7': third DEAE-cellulose chromatography

Yersinia pseudotuberculosis

437992, 437994