1.17.1.8: 4-hydroxy-tetrahydrodipicolinate reductase

This is an abbreviated version!
For detailed information about 4-hydroxy-tetrahydrodipicolinate reductase, go to the full flat file.

Word Map on EC 1.17.1.8

1.17.1.8

diaminopimelate

glutamicum

meso-diaminopimelate

aspartokinase

imine

dhdps

2,6-pyridinedicarboxylate

medicine

alpha,beta-unsaturated

2'-phosphate

s-2-aminoethyl-l-cysteine

aspartate-semialdehyde

drug development

agriculture

Reaction

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate

(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate

Synonyms

AbDHDPR, CRR1, DapB, DHDP reductase, DHDPR, DHPR, dihydrodipicolinate reductase, dihydrodipicolinate reductase-like protein, dihydrodipicolinic acid reductase, EC 1.3.1.26, MRSA-DHDPR, reductase, dihydrodipicolinate

ECTree

1 Oxidoreductases

1.17 Acting on CH or CH₂ groups

1.17.1 With NAD⁺ or NADP⁺ as acceptor

1.17.1.8 4-hydroxy-tetrahydrodipicolinate reductase

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Results	in table
8	Activating Compound
36207	AA Sequence
6	Application
1	CAS Registry Number
26	Cloned(Commentary)
65	Cofactor
16	Crystallization (Commentary)
18	General Information
61	Inhibitors
15	Ki Value [mM]
60	KM Value [mM]
3	Localization
31	Molecular Weight [Da]
39	Natural Substrates/ Products (Substrates)
76	Organism
11	Pathway
56	PDB ID
13	pH Optimum
3	pH Range
7	Protein Variants
24	Purification (Commentary)
13	Reaction
3	Reaction Type
59	Reference
3	Source Tissue
16	Specific Activity [micromol/min/mg]
2	Storage Stability
100	Substrates and Products (Substrate)
38	Subunits
71	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Temperature Stability [°C]
17	Turnover Number [1/s]

Purification

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Purification on EC 1.17.1.8 - 4-hydroxy-tetrahydrodipicolinate reductase

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9 entries

cells grown in M9 medium containing 2H, 15N, and 13C-labeled sources, collected, lysed, and protein purified with a QA52 anion exchange column and a Blue Trisacryl affinity column with gravity gradient chromatography, fractions with activity pooled and concentrated using an Amicon ultrafiltration device with YM10 membrane, desalted and loaded onto the Blue Trisacryl affinity column and eluted with a linear gradient of 0-2 M NaCl, 25 mM Tris buffer, pH 7.5, pooled, concentrated and buffer ecxchanged

Escherichia coli

696300

His-Trap column chromatography

Thermotoga maritima

Q9X1K8

687457

Ni-NTA agarose column chromatography

Pseudomonas aeruginosa

722411

Ni2+ affinity column chromatography and Superose 12 gel filtration

Staphylococcus aureus

723702

Ni2+-NTA affinity column chromatography and DEAE-Sepharose column chromatography

Staphylococcus aureus

Q5HG24

722272

overexpression in Escherichia coli from plasmid

2 entries

partial

2 entries

protein is purified using a QA52 anion exchange column and a Blue Trisacryl affinity column with gravity gradient chromatography

Escherichia coli

696300

purified from wild-type

Escherichia coli

390619

recombinant enzyme 5.9fold from Escherichia coli BL21(DE3) by ion-exchange and hydrophobic interaction chromatography, and gel filtration

Staphylococcus aureus

710760

recombinant enzyme expressed in Escherichia coli

Mycobacterium tuberculosis

390623