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1.17.4.1: ribonucleoside-diphosphate reductase

This is an abbreviated version!
For detailed information about ribonucleoside-diphosphate reductase, go to the full flat file.

Word Map on EC 1.17.4.1

Reaction

2'-deoxyribonucleoside 5'-diphosphate
+
thioredoxin disulfide
 +
H2O
=
ribonucleoside 5'-diphosphate
 +
thioredoxin

Synonyms

2'-deoxyribonucleoside-diphosphate:oxidized-thioredoxin 2'-oxidoreductase, ADP reductase, CDP reductase, class I ribonucleotide reductase, class I ribonulceotide reductase, class I RNR, class I RR, class Ia ribonucleotide reductase, class Ia RNR, class Ia RR, class Ib ribonucleotide reductase, class Ib RNR, class Ic ribonucleotide reductase, class II RNR, manganese-ribonucleotide reductase, Mn-RNR, More, mRR, NrdA, NrdB, NrdE, NrdF, nucleoside diphosphate reductase, p53-inducible ribonucleotide reductase, R2F, reductase, ribonucleoside diphosphate, ribonucleoside 5'-diphosphate reductase, ribonucleoside diphosphate reductase, ribonucleoside-diphosphate reductase subunit M2 B, ribonucleotide diphosphate reductase, ribonucleotide reductase, RIR1, RIR2, RNR, RNR1 rRibonucleoside-diphosphate reductase large chain 1, RNR2, UDP reductase

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.4 With a disulfide as acceptor
                1.17.4.1 ribonucleoside-diphosphate reductase

Purification

Purification on EC 1.17.4.1 - ribonucleoside-diphosphate reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Blue Sepharose, affinity resin
-
ammonium sulfate, Sephadex G-200, dATP-Sepharose
-
ammonium sulfate, Sephadex G-200, partially purified
-
chimeric R2 genes in which C-terminal sequences in Escherichia coli subunit R2 are replaced by C-terminal sequences from the mouse subunit R2
enzyme induced in Escherichia coli after infection with bacteriophage T4, streptomycin, ammonium sulfate, dATP-Sepharose
Tequatrovirus T4
-
gel filtration, ATP-agarose, partial purification
-
HSV type 2
Herpes simplex virus
-
isolation of beta-subunit NrdF using anion exchange chromatography to separate apo-/mismetalated-NrdFs from dimanganic-tyrosyl radical cofactor
-
low-salt precipitation, DEAE-cellulose, Supredex 200, Mono Q
M1 subunit from a mutant cell line of S49 T-lymphoma cells
-
native NrdF from strain GR536
-
native R2F subunit by 2',5'-ADP affinity and hydrophobic interaction chromatography, followed by two different steps of gel filtration, an further by anion exchange chromnatography for metallo-cofactor analysis
-
Novikoff ascites tumor cells, partial
-
overproducing strain
-
R1E subunit, ammonium sulfate, DEAE-Sepharose, dATP-Sepharose
-
R2F subunit, ammonium sulfate, DEAE-Sepharose, Mono Q, Superdex
-
recombinant B2 subunit, Sephadex G-25, DEAE Bio gel, Sephadex QAE-50
-
recombinant His-tagged alpha and beta subunits by affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nicke affinity chromatography and gel filtration
-
recombinant His-tagged ORF60 protein, subunit R2, from Escherichia coli BL21 (DE3) by metal affinity chromatography and gel filtration
-
recombinant His-tagged subunits R2 and R4 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant His6-tagged subunits hRRM1 and hRRM2 from Escherichia coli strain BL21(DE3)
-
recombinant His6-tagged subunits M2, p53R2, and M1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant NrdI, NdE, NrdF, and YmaB from Escherichia coli. The absorption spectra of the purified NrdENrdF complex exhibited characteristics of a Mn(III)2-Y radical center with 2 Mn/beta2 and 0.5 Y radical/beta2
-
recombinant R2F by anion exchange chromatography, gel filtration, and another different step of anion exchange chromatography to homogeneity
-
recombinant R2F from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
recombinant wild-type R1 and R2 subunits and F127Y, Y129F and F127Y/Y129F R2 mutant subunits
-
regenerating liver, dATP-Sepharose affinity chromatography
-
streptomycin sulfate, ammonium sulfate, partial purification
Herpes simplex virus
-
streptomycin, ammonium sulfate, DEAE-cellulose, hydroxylapatite, dATP-Sepharose
-
streptomycin, DEAE-Sepharose, Phenyl-Sepharose, Heparin-Sepharose, Sephacryl S-200
-
Y370F and Y370w mutant enzymes
-