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1.17.4.4: vitamin-K-epoxide reductase (warfarin-sensitive)

This is an abbreviated version!
For detailed information about vitamin-K-epoxide reductase (warfarin-sensitive), go to the full flat file.

Word Map on EC 1.17.4.4

Reaction

phylloquinol
+
a protein with a disulfide bond
=
phylloquinone
 +
a protein with reduced L-cysteine residues

Synonyms

EC 1.1.4.1, phylloquinone epoxide reductase, reductase, phylloquinone epoxide, vitamin K 2,3-epoxide reductase, vitamin K 2,3-epoxide reductase complex subunit 1, vitamin K 2,3-epoxide reductase complex subunit-1, vitamin K epoxid reductase, vitamin K epoxide reductase, vitamin K epoxide reductase complex subunit 1, vitamin K oxidoreductase, Vitamin K reductase, vitamin K-2,3-epoxide reductase, vitamin K-2,3-epoxide reductase subunit 1, vitamin K-epoxide reductase, vitamin K1 epoxide reductase, vitaminK epoxide reductase, VKOR, VKOR complex, VKORC1, VKORC1 variant 2, VKORC1-like 1, VKORC1L1, VKORC1v2, warfarin sensitive vitamin K 2,3-epoxide reductase, warfarin-sensitive vitamin K1 2,3-epoxide reductase

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.4 With a disulfide as acceptor
                1.17.4.4 vitamin-K-epoxide reductase (warfarin-sensitive)

Purification

Purification on EC 1.17.4.4 - vitamin-K-epoxide reductase (warfarin-sensitive)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Sepharose 6B, hydroxylapatite, microsomal epoxide hydrolase copurifies with vitamin KO reductase
-
DE52 cellulose column chromatography, Bio-Gel P-100 gel filtration, and agarose-glutathione column chromatography
-
MonoS, hydroxyapatite, DEAE-Sepharose, Sephacryl S-200, partially purified enzyme
-
native enzyme from liver microsomes by solubilization, pH 11, deoxycholate-Tris base microsome extraction procedure, immunoprecipitation, gel filtration, and protein A and protein G affinity chromatography
-
partially by microsome preparation
partially by microsome preparation by ultracentrifugation
partially, microsome preparation by ultracentrifugation
-
purification of a high-molecular-weight complex that is positive for thiol-disulfide oxidoreductase MdbA and vitamin K epoxide reductase VKOR in the C101A mutant
-
recombinant enzyme from Sf9 insect cell microsomes by ultracentrifugation, followed by HPC4 affinity chromatography in presence of DHPC and reconstitution in mixed detergent micelles, method optimization
-