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13500
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FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE
160000
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MoFe protein, estimation from Mo content, ultracentrifugation
168000
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MoFe protein, ultracentrifugation
180000
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MoFe protein, gel filtration
194000
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MoFe protein, gel filtration
200000 - 250000
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tungsten-substituted enzyme component, gel filtration
210000
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MoFe protein, gel filtration
219000
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MoFe protein, gel filtration
226000
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MoFe protein, gel filtration
227000
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MoFe protein, gel filtration
270000
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MoFe protein, ultracentrifugation
30800
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MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE
33500
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MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE
35000 - 40000
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Fe protein
36000
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2 * 36000, SDS-PAGE
40000
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Fe protein, ultracentrifugation
44000
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FeMo cofactor-NafY protein complex, gel filtration
46000
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NafY protein, gel filtration, sedimentation equilibrium centrifugation
55000
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MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE
56000
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Fe protein, gel filtration
57000
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MoFe protein: 2 * 59000 + 2 * 57000, alpha2beta2, SDS-PAGE
62000
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Fe protein, gel filtration
65000
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Fe protein, gel filtration
68200
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Fe protein, ultracentrifugation
74000
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Fe protein, gel filtration
160000 - 270000
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MoFe protein: various methods, overview
160000 - 270000
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MoFe protein: various methods, overview
160000 - 270000
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MoFe protein: various methods, overview
160000 - 270000
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MoFe protein: various methods, overview
160000 - 270000
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MoFe protein: various methods, overview
160000 - 270000
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MoFe protein: various methods, overview
160000 - 270000
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MoFe protein: various methods, overview
200000
-
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200000
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MoFe protein, ultracentrifugation
200000
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MoFe protein, ultracentrifugation
200000
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MoFe protein, gel filtration
216000
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MoFe protein, gel filtration
216000
Cyanobacterium sp.
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nitrogenase complex
220000
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MoFe protein, gel filtration
220000
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MoFe protein, gel filtration
220000 - 250000
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component I MoFe protein
220000 - 250000
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component I MoFe protein
220000 - 250000
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component I MoFe protein
230000
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MoFe protein
27500
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2 * 27500, SDS-PAGE
27500
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component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE
40000 - 74000
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Fe protein: various methods, overview
40000 - 74000
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Fe protein: various methods, overview
40000 - 74000
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Fe protein: various methods, overview
40000 - 74000
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Fe protein: various methods, overview
40000 - 74000
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Fe protein: various methods, overview
51000
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Fe protein, gel filtration
51000
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component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE
51000
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FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE
55000 - 60000
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component II Fe protein
55000 - 60000
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component II Fe protein
55000 - 60000
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component II Fe protein
59000
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MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE
59000
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MoFe protein: 2 * 59000 + 2 * 57000, alpha2beta2, SDS-PAGE
59000
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FeFe protein of iron-only enzyme: 2 * 59000 + 2 * 51000 + 2 * 13500, alpha2beta2gamma2, SDS-PAGE
60000
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Fe protein
60000
Cyanobacterium sp.
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Fe protein
60000
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component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE
60000
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MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE
63000
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Fe protein
64000
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Fe protein, gel filtration
64000
-
Fe protein, gel filtration
additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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additional information
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enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex
additional information
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enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex
additional information
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enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex
additional information
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enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex
additional information
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enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex
additional information
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enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex
additional information
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comparison of amino acid composition
additional information
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comparison of amino acid composition
additional information
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comparison of amino acid composition
additional information
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the nitrogenase activity is resolved into two components. The component 1 has a molecular size of approximately 250 kDa, as estimated by gel filtration, and sodium dodecyl sulfate-polyacrylamide gels reveals two predominant bands with molecular sizes near 57000 and 62000 Da, consistent with an alpha2beta2 tetramer as in eubacterial component 1 proteins. For the component 2 a molecular size of approximately 120000 Da is estimated by gel filtration, with a subunit molecular size near 31000 Da, indicating that the component 2 protein is a tetramer