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0.1 - 0.3
2-oxoglutarate semialdehyde
0.041
3-sulfopropanal
Km apparent value
0.0069 - 0.035
benzaldehyde
0.002 - 20.5
succinate semialdehyde
0.0011 - 0.03
Succinic semialdehyde
additional information
additional information
-
0.1
2-oxoglutarate semialdehyde
-
with NAD+ as cosubstrate, at pH 6.5 and 70°C
0.2
2-oxoglutarate semialdehyde
-
with NADP+ as cosubstrate, at pH 6.5 and 70°C
0.3
2-oxoglutarate semialdehyde
-
in 0.1 M HEPES/KOH (pH 6.5), at 70°C
0.0069
benzaldehyde
-
in the presence of NADP+, at 25°C and pH 8.5
0.0071
benzaldehyde
-
in the presence of NAD+, at 25°C and pH 8.5
0.011
benzaldehyde
-
recombinant wild type enzyme, in the presence of NADP+, TAPS buffer, at pH 8.5, at 30°C
0.011
benzaldehyde
-
His-tagged BADH, cosubstrate NADP+
0.013
benzaldehyde
-
native wild type enzyme, in the presence of NADP+, TAPS buffer, at pH 8.5, at 30°C
0.013
benzaldehyde
-
wild-type BADH, cosubstrate NADP+
0.033
benzaldehyde
-
native wild type enzyme, in the presence of NAD+, TAPS buffer, at pH 8.5, at 30°C
0.033
benzaldehyde
-
wild-type BADH, cosubstrate NAD+
0.035
benzaldehyde
-
recombinant wild type enzyme, in the presence of NAD+, TAPS buffer, at pH 8.5, at 30°C
0.035
benzaldehyde
-
His-tagged BADH, cosubstrate NAD+
0.0092
NAD+
-
in the presence of 10 mM Mg2+, at pH 7.5 and 37°C
0.033
NAD+
-
pH 7.8, 30°C
0.043
NAD+
wild-type, pH 8.0, 37°C
0.043
NAD+
wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C
0.107
NAD+
-
pH 8.0, 30°C
0.111
NAD+
-
recombinant wild type enzyme, in TAPS buffer, at pH 8.5, at 30°C
0.111
NAD+
-
His-tagged BADH, cosubstrate benzaldehyde
0.134
NAD+
-
native wild type enzyme, in TAPS buffer, at pH 8.5, at 30°C
0.134
NAD+
-
wild-type BADH, cosubstrate benzaldehyde
0.15
NAD+
mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C
0.15
NAD+
mutant N193A, pH 8.0, 37°C
0.21
NAD+
-
pH and temperature not specified in the publication
0.21
NAD+
pH not specified in the publication, 30°C
0.23
NAD+
Km apparent value
0.23
NAD+
recombinant His6-tagged enzyme, pH 7.0, 30°C
0.48
NAD+
wild-type, pH 8.0, 37°C
0.51
NAD+
pH 9.0, 25°C, recombinant mutant S157E
0.997
NAD+
-
in the presence of benzaldehyde, at 25°C and pH 8.5
1.12
NAD+
pH 8.5, 30°C, recombinant enzyme, with succinate semialdehyde
1.5
NAD+
pH 9.0, 25°C, recombinant mutant K154A
1.7
NAD+
pH 9.0, 25°C, recombinant wild-type enzyme
9.1
NAD+
-
in 0.1 M HEPES/KOH (pH 6.5), at 70°C
9.4
NAD+
-
mitochondria of non-synaptic origin
10
NAD+
mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C
10
NAD+
mutant K160A , pH 8.0, 37°C
11
NAD+
mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C
11
NAD+
mutant K160E , pH 8.0, 37°C
11.1
NAD+
-
at pH 6.5 and 70°C
0.004
NADP+
pH 8.5, 30°C, recombinant enzyme, with succinate semialdehyde
0.01
NADP+
-
at pH 6.5 and 70°C
0.034
NADP+
pH 9.0, 25°C, recombinant wild-type enzyme
0.0613
NADP+
-
in the absence of Mg2+, at pH 7.5 and 37°C
0.1
NADP+
recombinant His6-tagged enzyme, pH 7.0, 30°C
0.154
NADP+
-
pH 7.8, 30°C
0.22
NADP+
-
native wild type enzyme, in TAPS buffer, at pH 8.5, at 30°C
0.22
NADP+
-
wild-type BADH, cosubstrate benzaldehyde
0.287
NADP+
-
recombinant wild type enzyme, in TAPS buffer, at pH 8.5, at 30°C
0.287
NADP+
-
His-tagged BADH, cosubstrate benzaldehyde
0.35
NADP+
Km apparent value
0.39
NADP+
-
pH and temperature not specified in the publication
0.39
NADP+
pH not specified in the publication, 30°C
0.482
NADP+
wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C
1.1
NADP+
pH 9.0, 25°C, recombinant mutant S157E
2.4
NADP+
mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C
2.4
NADP+
mutant N193A, pH 8.0, 37°C
6.1
NADP+
pH 9.0, 25°C, recombinant mutant v
6.14
NADP+
-
in the presence of benzaldehyde, at 25°C and pH 8.5
26
NADP+
mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C
26
NADP+
mutant K160A , pH 8.0, 37°C
27
NADP+
mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C
27
NADP+
mutant K160E , pH 8.0, 37°C
0.002
succinate semialdehyde
pH 8.0, 60°C, cosubstrate: NAD+
0.0028
succinate semialdehyde
pH 9.0, 25°C, recombinant mutant S157E, with NAD+
0.0029
succinate semialdehyde
pH 8.0, 60°C, cosubstrate: NADP+
0.003
succinate semialdehyde
-
with NADP+ as cosubstrate, in the presence of 10 mM Mg2+, at pH 7.5 and 37°C
0.003
succinate semialdehyde
pH 8.5, 30°C, recombinant enzyme, with NADP+
0.003
succinate semialdehyde
pH 9.0, 25°C, recombinant mutant S157E, with NADP+
0.004
succinate semialdehyde
pH 9.0, 25°C, recombinant wild-type enzyme, with NAD+
0.007
succinate semialdehyde
pH 8.5, 30°C, recombinant enzyme, with NAD+
0.0073
succinate semialdehyde
-
pH 8.0, 30°C
0.008
succinate semialdehyde
pH 9.0, 25°C, recombinant wild-type enzyme, with NADP+
0.0133
succinate semialdehyde
-
with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C
0.017
succinate semialdehyde
-
-
0.02
succinate semialdehyde
pH 9.0, 25°C, recombinant mutant K154A, with NAD+
0.02
succinate semialdehyde
pH 9.0, 25°C, recombinant mutant K154A, with NADP+
0.022
succinate semialdehyde
mutant enzyme R428A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C
0.022
succinate semialdehyde
mutant R428A , cofactor NAD+, pH 8.0, 37°C
0.027
succinate semialdehyde
wild type enzyme, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C
0.027
succinate semialdehyde
wild-type, cofactor NAD+, pH 8.0, 37°C
0.04
succinate semialdehyde
-
-
0.047
succinate semialdehyde
wild type enzyme, with NADP+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C
0.047
succinate semialdehyde
wild-type, cofactor NADP+, pH 8.0, 37°C
0.054
succinate semialdehyde
-
pH 7.8, 30°C
0.12
succinate semialdehyde
mutant enzyme F138A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C
0.12
succinate semialdehyde
mutant F138A , cofactor NAD+, pH 8.0, 37°C
0.14
succinate semialdehyde
mutant enzyme E442A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C
0.14
succinate semialdehyde
mutant E442A, cofactor NAD+, pH 8.0, 37°C
0.16 - 0.18
succinate semialdehyde
-
either with NAD+ or with NADP+
0.22
succinate semialdehyde
mutant enzyme S425A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C
0.22
succinate semialdehyde
mutant S425A , cofactor NAD+, pH 8.0, 37°C
0.23
succinate semialdehyde
mutant enzyme D426A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C
0.23
succinate semialdehyde
mutant D426A , cofactor NAD+, pH 8.0, 37°C
0.77
succinate semialdehyde
mutant enzyme W141A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C
0.77
succinate semialdehyde
mutant W141A , cofactor NAD+, pH 8.0, 37°C
1.06
succinate semialdehyde
-
-
1.4
succinate semialdehyde
-
synaptosomes
1.5
succinate semialdehyde
-
mitochondria of non-synaptic origin
2.49
succinate semialdehyde
mutant enzyme R145A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C
2.49
succinate semialdehyde
mutant R145A , cofactor NAD+, pH 8.0, 37°C
2.6
succinate semialdehyde
mutant enzyme K92A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C
2.6
succinate semialdehyde
mutant K92A, cofactor NAD+, pH 8.0, 37°C
10.36
succinate semialdehyde
cofactor NAD+, pH not specified in the publication, 30°C
10.36
succinate semialdehyde
-
pH and temperature not specified in the publication, in presence of NAD+
20.5
succinate semialdehyde
cofactor NADP+, pH not specified in the publication, 30°C
20.5
succinate semialdehyde
-
pH and temperature not specified in the publication, in presence of NADP+
0.0011
Succinic semialdehyde
-
with NAD+ as cosubstrate, at pH 6.5 and 70°C
0.004
Succinic semialdehyde
-
with NADP+ as cosubstrate, at pH 6.5 and 70°C
0.03
Succinic semialdehyde
-
in 0.1 M HEPES/KOH (pH 6.5), at 70°C
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics, kinetic analysis
-
additional information
additional information
-
Michaelis-Menten kinetics, kinetic analysis
-
additional information
additional information
Michaelis-Menten kinetics, steady-state kinetics of cce4228 wild-type and mutants enzymes
-