1.2.1.27: methylmalonate-semialdehyde dehydrogenase (CoA-acylating)
This is an abbreviated version!
For detailed information about methylmalonate-semialdehyde dehydrogenase (CoA-acylating), go to the full flat file.
Word Map on EC 1.2.1.27
-
1.2.1.27
-
lysosomotropic
-
3-hydroxyisobutyrate
-
coa-dependent
-
mississippi
-
propionyl-coa
-
aldh6a1
-
3-hydroxypropionic
- 1.2.1.27
-
lysosomotropic
- 3-hydroxyisobutyrate
-
coa-dependent
-
mississippi
- propionyl-coa
- aldh6a1
-
3-hydroxypropionic
Reaction
Synonyms
Aldh6a1, dddC, FG99_15390, KES23460, malonate-semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase protein, methylmalonate-semialdehyde dehydrogenase, methylmalonic acid semialdehyde dehydrogenase, MGG_01606, MMSADH, MMSDH, MoMSDH, MSDH, NAD-dependent malonate-semialdehyde dehydrogenase, OdoMMSDH, OsALDH6, PA3570, SSO1218
ECTree
1.2.1.27 methylmalonate-semialdehyde dehydrogenase (CoA-acylating)Advanced search results
results (
results (KM Value
KM Value on EC 1.2.1.27 - methylmalonate-semialdehyde dehydrogenase (CoA-acylating)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
additional information
additional information
-
Km-value of CoA for R301L mutant enzyme under steady-state conditions with propionaldehyde is above 1 mM at pH 8.2 and 30°C
-
0.06
2-Methyl-3-oxopropanoate
-
50 mM KP04 pH 8.2, 30°C, 0.5 mM 2-methyl-3-oxopropanoate, 12 mM NAD+, 0.5 mM CoA
0.085
CoA
-
wild type MSDH, steady-state conditions with propionaldehyde, pH 8.2 and 30°C
0.12
CoA
-
50 mM potassium phosphate pH 8.2, 30°C, 0.5 mM 2-methyl-3-oxopropanoate, 12 mM NAD+, 0.5 mM CoA
0.12
CoA
-
wild type MSDH, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C
0.33
CoA
-
R124L mutant enzyme, steady-state conditions with propionaldehyde, pH 8.2 and 30°C
0.57
CoA
-
R124L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C
0.62
CoA
-
R301L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C
0.006
methylmalonate semialdehyde
-
R124L mutant enzyme, steady-state conditions, pH 8.2 and 30°C
0.021
methylmalonate semialdehyde
V229G/H226P/G225 insertion, pH 8.2 and 30°C
0.023
methylmalonate semialdehyde
-
R301L mutant enzyme, steady-state conditions, pH 8.2 and 30°C
0.027
methylmalonate semialdehyde
V229G/G225 insertion, pH 8.2 and 30°C
0.06
methylmalonate semialdehyde
-
wild type MSDH, steady-state conditions, pH 8.2 and 30°C
0.215
methylmalonate semialdehyde
V229G/Y252L/V253I/G225 insertion, pH 8.2 and 30°C
0.033
NAD+
-
wild type MSDH, steady-state conditions with propionaldehyde, pH 8.2 and 30°C
0.038
NAD+
-
R124L mutant enzyme, steady-state conditions with propionaldehyde, pH 8.2 and 30°C
0.06
NAD+
-
R124L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C
0.12
NAD+
-
R301L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C
2.3
NAD+
-
50 mM potassium phosphate pH 8.2, 30°C, 0.5 mM 2-methyl-3-oxopropanoate, 12 mM NAD+, 0.5 mM CoA
2.3
NAD+
-
wild type MSDH, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C
9.3
propionaldehyde
-
R124L mutant enzyme, steady-state conditions, pH 8.2 and 30°C
9.8
propionaldehyde
-
wild type MSDH, steady-state conditions, pH 8.2 and 30°C
