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1.2.1.30: carboxylate reductase (NADP+)

This is an abbreviated version!
For detailed information about carboxylate reductase (NADP+), go to the full flat file.

Word Map on EC 1.2.1.30

Reaction

an aromatic aldehyde
+
NADP+
 +
AMP
 +
diphosphate
=
an aromatic acid
 +
NADPH
 +
H+
 +
ATP

Synonyms

aromatic acid reductase, aryl aldehyde:NADP+ oxidoreductase, aryl-aldehyde dehydrogenase (NADP+), aryl-aldehyde oxidoreductase, ATP/NADPH-dependent carboxylic acid reductase, CAR, carboxylate reductase, carboxylate reductases, Carboxylic acid reductase, kaCAR, mab3CAR, maCAR, mmCAR, mpCAR, msCAR, naCAR, NcCAR, niCAR, noCAR, tpCAR, type I CAR, type III CAR

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.30 carboxylate reductase (NADP+)

Cloned

Cloned on EC 1.2.1.30 - carboxylate reductase (NADP+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli resulting in decreased activity due to a lack of post-translational phosphopantetheinylation of a serine group in the recombinant CAR that is necessary for activity. Coexpression with the recombinant phosphopantetheinyl transferase (PPTase) from Bacillus subtilis (Sfp) or cell-free extracts from Nocardia iowensis containing native PPTase converted niCAR to its holoenzyme state, increases the enzyme activity 20fold. Recombinant enzyme expression in Saccharomyces cerevisiae and Saccharomyces pombe and an engineered aldehyde-accumulating Escherichia coli strain for de novo production of vanillin from glucose
expressed in Escherichia coli BL21(DE3) cells
plasmids pHAT305(Placcar), pPV2.83(Placcar Placnpt), pPV2.84(Placcar Placgdh) or pPV2.85(Placcar Plac npt gdh) expressed in Escherichia coli BL21-CodonPlus(DE3)-RP cells
recombinant car expressed in Escherichia coli BL21-CodonPlus(DE3)-RP/pHAT305 and in Escherichia coli BL21-CodonPlus(DE3)-RP/pPV1.184 cells
-
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain MG1655
sequence comparisons and phylogenetic analysis, recombinant coexpression of His-tagged enzyme in Escherichia coli
sequence comparisons and phylogenetic analysis, recombinant coexpression of His-tagged enzyme with Escherichia coli phosphopantetheinyl transferase, EcPPTase, in Escherichia coli strain MG1655 RARE
-