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1.2.1.30: carboxylate reductase (NADP+)

This is an abbreviated version!
For detailed information about carboxylate reductase (NADP+), go to the full flat file.

Word Map on EC 1.2.1.30

Reaction

an aromatic aldehyde
+
NADP+
+
AMP
+
diphosphate
=
an aromatic acid
+
NADPH
+
H+
+
ATP

Synonyms

aromatic acid reductase, aryl aldehyde:NADP+ oxidoreductase, aryl-aldehyde dehydrogenase (NADP+), aryl-aldehyde oxidoreductase, ATP/NADPH-dependent carboxylic acid reductase, CAR, carboxylate reductase, carboxylate reductases, Carboxylic acid reductase, kaCAR, mab3CAR, maCAR, mmCAR, mpCAR, msCAR, naCAR, NcCAR, niCAR, noCAR, tpCAR, type I CAR, type III CAR

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.30 carboxylate reductase (NADP+)

Purification

Purification on EC 1.2.1.30 - carboxylate reductase (NADP+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by a combination of Mono-Q, Reactive Green 19 agarose affinity chromatography, and hydroxyapatite chromatography
Nocadia sp.
-
by gel filtration
-
by precipitation of nucleic acids with protamine sulfate, ammonium sulphate fractionation, adsorption on calcium-phosphate gel and chromatography on TEAE-cellulose and on hydroxylapatite
-
nickel affinity column chromatography
recombinant enzyme with his-tag
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and desalting gel filtration
recombinant His-tagged enzyme from Escherichia coli strain MG1655 RARE by nickel affinity chromatography and desalting gel filtration
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain MG1655 by nickel affinity chromatography