1.2.1.32: aminomuconate-semialdehyde dehydrogenase

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For detailed information about aminomuconate-semialdehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.32

1.2.1.32

catechols

putida

meta-cleavage

hydratase

4-oxalocrotonate

2-aminophenols

toluate

m-cresol

3-methylcatechol

4-hydroxy-2-oxovalerate

nitrobenzene

comamonas

xylf

extradiol

chloroaromatics

isofunctional

Reaction

Synonyms

2-aminomuconate semialdehyde dehydrogenase, 2-aminomuconate-6-semialdehyde dehydrogenase, 2-aminomuconic 6-semialdehyde dehydrogenase, 2-AMS dehydrogenase, 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate dehydrogenase, 2-hydroxymuconate semialdehyde dehydrogenase, 2-hydroxymuconic 6-semialdehyde dehydrogenase, 2-hydroxymuconic acid semialdehyde dehydrogenase, 2-hydroxymuconic semialdehyde dehydrogenase, aldehyde dehydrogenase, ALDH12, Aldh8a1, alpha-aminomuconic epsilon-semialdehyde dehydrogenase, alpha-hydroxymuconic epsilon-semialdehyde dehydrogenase, AMDH, aminomuconate semialdehyde dehydrogenase, aminomuconate-semialdehyde dehydrogenase, AMSDH, dehydrogenase, 2-hydroxymuconate semialdehyde, dehydrogenase, aminomuconate semialdehyde, HMSD-2, nbaE

ECTree

1 Oxidoreductases

1.2 Acting on the aldehyde or oxo group of donors

1.2.1 With NAD⁺ or NADP⁺ as acceptor

1.2.1.32 aminomuconate-semialdehyde dehydrogenase

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Results	in table
1	Activating Compound
339	AA Sequence
1	Application
1	CAS Registry Number
13	Cloned(Commentary)
21	Cofactor
1	Crystallization (Commentary)
7	General Information
22	Inhibitors
2	kcat/KM [mM/s]
2	Ki Value [mM]
38	KM Value [mM]
12	Molecular Weight [Da]
25	Natural Substrates/ Products (Substrates)
152	Organism
9	Pathway
2	PDB ID
6	pH Optimum
2	pH Range
1	pI Value
10	Protein Variants
14	Purification (Commentary)
3	Reaction
7	Reaction Type
42	Reference
4	Source Tissue
11	Specific Activity [micromol/min/mg]
1	Storage Stability
127	Substrates and Products (Substrate)
12	Subunits
34	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
5	Turnover Number [1/s]

Purification

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Purification on EC 1.2.1.32 - aminomuconate-semialdehyde dehydrogenase

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2 entries

ammonium sulfate fractionation, chromatography on DE52 cellulose, chromatography on DEAE-cellulofine, chromatography on DEAE-toyopearl

Bordetella sp.

672682

ammonium sulfate precipitation followed by 3 chromatography steps

Bordetella sp.

672682

by nickelnitrilotriacetic acid affinity chromatography

Burkholderia cepacia

669112

erroneous identification as benzaldehyde dehydrogenase corrected

Pseudomonas putida

390225

erroneously identified as benzaldehyde dehydrogenase

Pseudomonas putida

389581, 390259

Ni-affinity chromatography

Burkholderia cepacia

669112

Ni-NTA column chromatography

partial

recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli

Pseudomonas fluorescens

Q83V33

743338