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1.2.1.50: long-chain acyl-protein thioester reductase

This is an abbreviated version!
For detailed information about long-chain acyl-protein thioester reductase, go to the full flat file.

Word Map on EC 1.2.1.50

Reaction

a long-chain aldehyde
+
[protein]-L-cysteine
+
NADP+
=
a [protein]-S-(long-chain fatty acyl)-L-cysteine
+
NADPH
+
H+

Synonyms

acyl coenzyme A reductase, acyl-CoA reductase, AmFAR1, CER4, CpFAS1-R, FACoAR, FAR1, FAR2, FAR3, FAR6, FAR8, fatty acyl-CoA reductase, fatty acyl-CoA reductase 1, fatty acyl-CoA reductase 2, fatty acyl-CoA reductase 3, fatty acyl-CoA reductase 6, fatty acyl-CoA reductase 8, long-chain-fatty-acyl-CoA reductase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.50 long-chain acyl-protein thioester reductase

Cloned

Cloned on EC 1.2.1.50 - long-chain acyl-protein thioester reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of CpFAS1-R domain in a pMAL-c2x vector with maltose binding protein-fusion protein and expression in Escherichia coli
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
-
heterologous expression of pYES2.1 plasmid harbouring AmFAR1 in Saccharomyces cerevisiae strain INVSc1
into the vector pET15b for expression in Escherichia coli Rosetta cells
overexpression in Escherichia coli
plasmid pHisMBPMarFAR harbouring the sequence of hypothetical protein Maqu_2220 added by His-tag and maltose binding protein transformed to Escherichia coli strain Rosetta (DE3) for heterologous protein expression
Marinobacter nauticus
-
plasmid pPCRMALD8 containing the FACoAR from Marinobacter aquaeolei VT8 with an N-terminal maltose binding protein fusion and a C-terminal His-tag transformed into the Escherichia coli TB1 strain for protein expression
Marinobacter nauticus
-