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1.2.1.65: salicylaldehyde dehydrogenase

This is an abbreviated version!
For detailed information about salicylaldehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.65

Reaction

Salicylaldehyde
+
NAD+
 +
H2O
=
salicylate
 +
NADH
 + 2 H+

Synonyms

NAD+-dependent salicylaldehyde dehydrogenase, NahF, NahV, SAL dehydrogenase, SALD, SALDan, sALDH, salicylaldehyde dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.65 salicylaldehyde dehydrogenase

Cloned

Cloned on EC 1.2.1.65 - salicylaldehyde dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli and Pseudomonas aeruginosa
-
mutant strains, lacking induction of enzyme synthesis by naphthalene growth media
-
nahF gene subcloned into the pET28a(TEV) vector and the recombinant protein with 6xHis tag at the N-terminaus overexpressed in Escherichia coli BL21 Arctic Express (DE3) cells
-
nahF inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3)
nahV inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3)
SALDH, DNA and amino acid sequence determination and analysis, the gene for SALDH is probably located on the chromosome in strains C4, C5 and C6, sequence comparisons and phylogenetic analysis
sequence comparisons and phylogenetic tree, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli