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1.2.1.79: succinate-semialdehyde dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about succinate-semialdehyde dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.2.1.79

Reaction

succinate semialdehyde
+
NADP+
 +
H2O
=
succinate
 +
NADPH
 + 2 H+

Synonyms

AbSSADH, ALDH21, all3556, ApSSADH, gabD, GabD1, NADP+-dependent SSADH, NADP+-dependent succinic semialdehyde dehydrogenase, NADP-dependent succinic semialdehyde dehydrogenase, PpSSALDH, slr0370, Sp2771, SpSSADH, SSADH, SSADH-II, SSALDH, SSO1842, succinic semialdehy de dehydrogenase, succinic semialdehyde dehydrogenase, SYNPCC7002_A2771, SySSADH

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.79 succinate-semialdehyde dehydrogenase (NADP+)

Cloned

Cloned on EC 1.2.1.79 - succinate-semialdehyde dehydrogenase (NADP+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
gene all3556, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain BL21(DE3)
gene gabD, recombinant expression in Corynebacterium glutamicum strain KCTC 1857, coexpression with 5-aminovalerate transaminase (davT, EC 2.6.1.48) from Pseudomonas putida, 4-aminobutyrate-2-oxoglutarate transaminase (gabT, EC 2.6.1.19) from Corynebacterium glutamicum, and glutarate semialdehyde dehydrogenase (gabD, EC 1.2.1.20) from Pseudomonas putida, as well as N-terminal His6-tagged lysine 2-monooxygenase (davB, EC 1.13.12.2) from Pseudomonas putida
gene gabD, recombinant expression of GST-tagged enzyme in Escherichia coli strain DH5alpha
gene ssadh, recombinant expression in Escherichia coli strain BL21(DE3)
insertion of the full length Sp2771 gene into pET28b vector with an N-terminal His-6 tag and expression in Escherichia coli (BL21/DE3) strain
N-terminal His-tagged SySSADH expressed in Escherichia coli B834 (DE3) methionine auxotroph cells
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21/DE3
recombinant expression of N-terminally His-tagged SySSADH (residues Met1 to Lys454) in Escherichia coli methionine-auxotrophic strain B834(DE3), recombinant expression of His-tagged enzyme mutants in Escherichia coli strain coli BL21(DE3). Juxtaposition of the N- and C-domains generates an active site tunnel between the two domains that is accessible from both ends. The catalytic residues are located in the middle of the tunnel. A nucleophile Cys262 is located in the catalytic loop between alphaa8 and beta11 of the C-domain, and a general base Glu228 is located in an interdomain-connecting loop between beta9 and beta10. Dimerization is mediated largely by N-domain alpha7 and the three antiparallel beta-strands (beta3, beta4, beta17) protruding from the N- and C-domains