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1.2.1.88: L-glutamate gamma-semialdehyde dehydrogenase

This is an abbreviated version!
For detailed information about L-glutamate gamma-semialdehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.88

Reaction

L-glutamate 5-semialdehyde
+
NAD+
 +
H2O
=
L-glutamate
 +
NADH
 +
H+

Synonyms

1-pyrroline dehydrogenase, aldehyde dehydrogenase 12, ALDH12, Aldh4a1, dehydrogenase, 1-pyrroline-5-carboxylate, DELTA1-pyrroline-5-carboxylate dehydrogenase, delta1-pyrroline-5-carboxylate synthetase, DR_0813, EC 1.5.1.12, GSAL dehydrogenase, GSALDH, l-glutamate-gamma-semialdehyde dehydrogenase, L-pyrroline-5-carboxylate-NAD+ oxidoreductase, LRRP Ba1-651, NAD+-dependent glutamate gamma-semialdehyde dehydrogenase, OsALDH12, OsALDH18-2, P5C dehydrogenase, P5C synthase, P5C-DH, P5CD, P5CDH, P5CDH1, P5CS, PDH, PpALDH12, proline/P5C dehydrogenase, Put2, PutA, pyrroline-5-carboxylate dehydrogenase, pyrroline-5-carboxylate synthase, pyrroline-5-carboxylic acid dehydrogenase, Tc00.1047053510943.50, YcgN, ZmALDH12

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.88 L-glutamate gamma-semialdehyde dehydrogenase

Crystallization

Crystallization on EC 1.2.1.88 - L-glutamate gamma-semialdehyde dehydrogenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
space group P21 with eight molecules in the asymmetric unit
Halalkalibacterium halodurans
the substrate-free form displays multiple active site conformations. Protein forms a trimer-of-dimers hexamer in solution
complexed with glutarate, succinate, malonate, glyoxylate, and acetate
apoenzyme and in complex with NAD+, to 1.95 and 2.17 A resolution, respectively
PutA with the following ligands bound in the GSALDH active site: NADH, D-proline, trans-4-hydroxy-D-proline, cis-4-hydroxy-D-proline, L-proline, and trans-4-hydroxy-L-proline, sitting drop vapor diffusion method, using 19% (w/v) PEG-3350, 0.2 M ammonium sulfate, 0.1 M magnesium chloride, 0.1 M HEPES (pH 8.0), and 0.1 M sodium formate
mutants R100A crystallizes in the same lattice as wild-type, mutant R100A/K104A/R111A crystaLLIZES IN space group P1
unligandend form and in complex with NAD+, NADPH, and with product glutamate
-
purified ZmALDH12 in complex with NAD+, sitting drop vapour diffusion method, mixing of 2 mg/ml protein in 50 mM Tris, pH 7.8, 50 mM NaCl, 0.5 mM tris(2-caboxyethyl)phosphine (TCEP), 1 mM NAD+, and 5% v/v glycerol with a crystallization solution containing 0.09 M sodium nitrate, sodium phosphate, and ammonium sulfate, and a precipitant mixture containing 25% v/v 2-methyl-2,4-pentanediol, 25% w/v PEG 1000, and 25% w/v polyethylene glycol 3350, and 0.1 M Tris/bicine pH 8.5, in a 1:1 ratio v/v, X-ray diffraction structure determination and analysis at 2.20 A resolution, structure modeling using molecular replacement and a search model derived from a betaine ALDH (PDB ID 4MPY)