1.2.3.6: pyruvate oxidase (CoA-acetylating)
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For detailed information about pyruvate oxidase (CoA-acetylating), go to the full flat file.
Reaction
Synonyms
pyruvate oxidase, SpxB
ECTree
1.2.3.6 pyruvate oxidase (CoA-acetylating)Advanced search results
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Engineering on EC 1.2.3.6 - pyruvate oxidase (CoA-acetylating)
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
G389D
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spxB2 mutant, reduces H2O2 production to approximately that of a DELTAspxB null mutant. SpxB2 mutant shows an opaque colony phenotype and decreased production of H2O2
P449L
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spxB4 mutant, reduces H2O2 production to approximately that of a DELTAspxB null mutant. SpxB4 mutant shows an opaque colony phenotype and decreased production of H2O2
additional information
additional information
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deletion of the spxB gene. no pyruvate oxidase activity and no inhibition of Staphylococcus growth
additional information
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cis-acting mutations in spxB decrease H2O2 production. SpxB1 mutant with a 7 bp deletion (Datagaga) of one repeat unit of an adjacent direct repeat centres 136 bp upstream from the translation start codon of SpxB. SpxB1 mutant shows an opaque colony phenotype and decreased production of H2O2. SpxB transcript is greatly reduced (14fold) in the spxB1 mutant compared with parent strain D39. DELTAspxB deletion/insertion mutant, which forms large, opaque colonies on TSA plates similar to those of the spxB2 mutant, produces a minimal basal level of H2O2, and synthesizes capsule
additional information
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knockout of the spxB gene in strain D39 abolishes spontaneous transformation. Significant reduced expression levels of comC and recA as well as DNA release from bacterial cells during the early growth phase, coincident with the time of spontaneous competence in the parent strain. Supplementation with competencestimulating peptide 1 restores transformation. Neither supplementation with competencestimulating peptide 1 nor supplementation with the SpxB products H2O2 and acetate alteres DNA release. Supplementation of the parent strain with catalase does not reduce DNA release significantly
additional information
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deletion of the spxB gene. no pyruvate oxidase activity and no inhibition of Staphylococcus growth
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additional information
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deletion of the spxB gene. no pyruvate oxidase activity and no inhibition of Staphylococcus growth
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