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1.2.4.1: pyruvate dehydrogenase (acetyl-transferring)

This is an abbreviated version!
For detailed information about pyruvate dehydrogenase (acetyl-transferring), go to the full flat file.

Word Map on EC 1.2.4.1

Reaction

pyruvate
+
[dihydrolipoyllysine-residue acetyltransferase] lipoyllysine
=
[dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine
 +
CO2

Synonyms

aceE, AcoA, CTHT_0006350, CTHT_0069820, dehydrogenase, pyruvate, E1 component of pyruvate dehydrogenase, E1 component of pyruvate dehydrogenase multienzyme complex, E1 component of the pyruvate dehydrogenase multienzyme complex, E1 component subunit alpha, E1 component subunit beta, E1alpha, E1ec, E1p, IAR4, MAB1, MdeB, mitochondrial pyruvate dehydrogenase, More, MtPDC, OsI_14647, OsI_31986, Pda1, PDC, PDH, PDH E1alpha, PDH subunit E1-beta, PDH-A1, PDHa, PdhA1, PDHA1a, PdhB, PDHC, PDHc E1, PDHc-E1, PdhE, PDHE1alpha, PdhH, PH2, pyruvate decarboxylase, pyruvate dehydrogenase, pyruvate dehydrogenase complex, pyruvate dehydrogenase complex E1 component, pyruvate dehydrogenase complex,, pyruvate dehydrogenase E1, pyruvate dehydrogenase E1 alpha subunit, pyruvate dehydrogenase E1 component, pyruvate dehydrogenase E1 component subunit beta, pyruvate dehydrogenase multienzyme complex, pyruvate dehydrogenase multienzyme complex E1, pyruvate dehydrogenase, E1, pyruvate:NAD oxidoreductase, pyruvate:[dihydrolipoyllysine-residue acetyltransferase]-lipoyllysine 2-oxidoreductase (decarboxylating, acceptor-acetylating), pyruvic acid dehydrogenase, pyruvic dehydrogenase, thiamin diphosphate-dependent pyruvate dehydrogenase, thiamin-dependent pyruvate dehydrogenase, thiamine diphosphate-dependent 2-oxo acid decarboxylase, VEG220, Vegetative protein 220

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.4 With a disulfide as acceptor
                1.2.4.1 pyruvate dehydrogenase (acetyl-transferring)

Specific Activity

Specific Activity on EC 1.2.4.1 - pyruvate dehydrogenase (acetyl-transferring)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0048
-
Y177A mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate 2alpha-hydroxyethyl-thiamin diphosphate
0.00574
in cells grown on 50 mM glucose and 2 mM acetate, at 22°C
0.00671
-
Y177F mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate 2alpha-hydroxyethyl-thiamin diphosphate
0.0098
-
wild-type enzyme, 2,6-dichlorophenolindophenol assay, substrate 2alpha-hydroxyethyl-thiamin diphosphate
0.011
in cells grown on 50 mM glycerol and 2 mM acetate, at 22°C
0.0162
-
recombinant His-tagged-alpha2,beta2 tetramer, assay with 2-hydroxyethylidene-thiamine diphosphate and 2,6-dichlorphenolindophenol
0.0269
-
recombinant His-tagged-alpha2,beta2 tetramer, assay with pyruvate and 2,6-dichlorphenolindophenol
0.063
-
decarboxylation in the absence of K3Fe(CN)6 as artificial electron acceptor
0.087
-
Y177A mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate pyruvate
0.13
-
recombinant alpha2beta2 tetramer
0.1416
-
decarboxylation in the presence of K3Fe(CN)6 as artificial electron acceptor
0.145
-
recombinant alpha2,His-tagged-beta2 tetramer, K3Fe(CN)6 as artificial electron acceptor
0.17
wild-type, 30°C, pH not specified in the publication
0.18
-
recombinant alpha2,beta2 tetramer with six histidine residues attached to the N-terminus
0.187
-
recombinant His-tagged-alpha2,beta2 tetramer, K3Fe(CN)6 as artificial electron acceptor
0.216
-
Y177F mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate pyruvate
0.367
-
recombinant alpha2,His-tagged-beta2 tetramer, assay with pyruvate and 2,6-dichlorphenolindophenol
0.385
-
purified recombinant wild-type E1, with 2,6-dichlorophenolindophenol as electron acceptor
0.445
-
wild-type enzyme, 2,6-dichlorophenolindophenol assay, substrate pyruvate
10.9
-
recombinant alpha2,His-tagged-beta2 tetramer, reduction of NAD+, overall reaction
14.4
-
recombinant His-tagged-alpha2,beta2 tetramer, reduction of NAD+, overall reaction
15.8
-
reduction of NAD+, overall reaction
1791
-
activity after reassociation of the purified enzyme with EC 2.3.1.12 and EC 1.8.1.4
28.35
-
purified recombinant wild-type E1, with NAD+ as electron acceptor
3.26
-
recombinant enzyme from testis
55
-
recombinant enzyme from Haloferax volcanii
8.13
-
recombinant enzyme from liver
87
pH 6.7, 30°C
91
-
recombinant enzyme from Escherichia coli