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1.2.7.1: pyruvate synthase

This is an abbreviated version!
For detailed information about pyruvate synthase, go to the full flat file.

Word Map on EC 1.2.7.1

Reaction

pyruvate
+
CoA
 +
oxidized ferredoxin
=
acetyl-CoA
 +
CO2
 +
reduced ferredoxin
 + 2 H+

Synonyms

2-ketobutyrate synthase, 2-oxobutyrate (methylviologen), 2-oxobutyrate synthase (benzylviologen), 2-oxobutyrate-ferredoxin oxidoreductase, alpha-ketobutyrate synthase, alpha-ketobutyrate-ferredoxin oxidoreductase, AP120, Ape2126/2128, bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase, EC 1.2.7.2, Moth_0064, NifJ1, OFOR, PFO, PFO A, PFOR, Pfor1, PforA, PFR, PFR1, POR, PorEDABG, pyruvate ferredoxin oxidoreductase, pyruvate-ferredoxin oxidoreductase, pyruvate:Fd oxidoreductase, pyruvate:ferredoxin oxidoreductase, pyruvate:ferredoxin oxidoreductase A, synthase, 2-oxobutyrate, TKV_c04340, Tsac_0046, TTE0445

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.7 With an iron-sulfur protein as acceptor
                1.2.7.1 pyruvate synthase

Specific Activity

Specific Activity on EC 1.2.7.1 - pyruvate synthase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.04
-
pH 7.5, 65°C, cell extract
0.06
-
pH 7.5, 65°C, cell extract
0.13
-
50°C, pH not specified in the publication, enzyme from lactate-grown cells
0.3
pH 8.6, 37°C, substrate: indol-3-pyruvate
0.4
-
using cytochrome c as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C
0.7
-
pH 7.5, 65°C, cell extract
1.8
acceptor methylene blue, pH 7.5, 66°C
12
-
using benzyl viologen as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C
2.3
-
using FMN as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C
20
-
pH 8.4, 80°C
20.2
purified enzyme, pH 8.4, 80°C
27.2
acceptor ferredoxin, pH 7.5, 66°C
3.46
-
pH 7.6, 70°C
3.6
pH 8.6, 37°C, substrate: 2-oxobutyrate
3.7
-
using methyl viologen as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C
4.5
-
using spinach ferredoxin 1 as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C
4.7
-
using methylene blue as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C
40
-
pH 7.5, 25°C
6.3
-
using Chlamydomonas reinhardtii ferredoxin 1 as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C
6.5
pH 8.6, 37°C, substrate: oxaloacetate
7.4
pH 8.6, 37°C, substrate: pyruvate
72
acceptor methyl viologen, pH 9, 40°C
78
Q56317; 05650; Q56316; O05651
pH 8.4, 80°C