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1.2.7.8: indolepyruvate ferredoxin oxidoreductase

This is an abbreviated version!
For detailed information about indolepyruvate ferredoxin oxidoreductase, go to the full flat file.

Word Map on EC 1.2.7.8

Reaction

(indol-3-yl)pyruvate
+
CoA
+ 2 oxidized ferredoxin =
S-2-(indol-3-yl)acetyl-CoA
+
CO2
+ 2 reduced ferredoxin + 2 H+

Synonyms

3-(indol-3-yl)pyruvate synthase (ferredoxin), arylpyruvate-ferredoxin oxidoreductase, hydroxyphenylpyruvate oxidase, indolepyruvate oxidase, indolepyruvate-ferredoxin oxidoreductase, indolepyruvate:Fd oxidoreductase, IOR, phenylpyruvate oxidase, VOR

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.7 With an iron-sulfur protein as acceptor
                1.2.7.8 indolepyruvate ferredoxin oxidoreductase

Purification

Purification on EC 1.2.7.8 - indolepyruvate ferredoxin oxidoreductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography, 74fold
-
purification under extremely anaerobic conditions in presence of 2mM dithiothreitol and 2mM sodium dithionite to protect against oxygen attack, and also 1mM MgSO4,1mM thiamine diphosphate and 0.2M NaCl, additives which have been found to stabilize
-
under extremely anaerobic conditions with all buffers containing 2 mM dithiothreitol and 2 mM dithiothreitol. Q Sepharose column chromatography, hydroxyapatite column chromatography, Blue Sepharose column chromatography, and Superdex 200 gel filtration
-