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1.20.4.4: arsenate reductase (thioredoxin)

This is an abbreviated version!
For detailed information about arsenate reductase (thioredoxin), go to the full flat file.

Word Map on EC 1.20.4.4

Reaction

arsenate
+
thioredoxin
=
arsenite
+
thioredoxin disulfide
+
H2O

Synonyms

ARS, ArsC, ArsC1, ArsC1', ArsC3, arsenate reductase, Bs_ArsC, pI258 arsenate reductase, plasmid pI258 arsenate reductase, Sa_ArsC, Strop634, thioredoxin-arsenate reductase complex, thioredoxin-coupled arsenate reductase, Trx-coupled arsenate reductase

ECTree

     1 Oxidoreductases
         1.20 Acting on phosphorus or arsenic in donors
             1.20.4 With disulfide as acceptor
                1.20.4.4 arsenate reductase (thioredoxin)

Purification

Purification on EC 1.20.4.4 - arsenate reductase (thioredoxin)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by fast protein liquid chromatography
C10S/C15A/C89L mutant and C10S/C15A/C82S mutant purified by Ni2+-NTA Superflow
gel filtration, SDS-PAGE
ion-exchange chromatography
mutant is purified on Ni2+-NTA Superflow
recombinant protein
wild-type ArsC and the Cys mutants, gel filtration more than 97% pure