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1.3.1.24: biliverdin reductase

This is an abbreviated version!
For detailed information about biliverdin reductase, go to the full flat file.

Word Map on EC 1.3.1.24

Reaction

bilirubin
+
NAD(P)+
=
Biliverdin
 +
NAD(P)H
 +
H+

Synonyms

biliverdin IXalpha reductase, biliverdin IXbeta reductase, biliverdin reductase, biliverdin reductase A, biliverdin reductase B, Biliverdin reductase-A, biliverdin-IXalpha reductase, BLVR subtype B, BLVRA, BLVRB, BV reductase, BVR, BVR-A, BVR-B, BVRA, BVRB, F-BVR, F420H2-dependent biliverdin reductase, hBVR, hBVR-A, reductase, biliverdin, Rv2074, slr1784

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.24 biliverdin reductase

Cloned

Cloned on EC 1.3.1.24 - biliverdin reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
biliverdin reductase cDNA cloned into pUC18, used for expression and purification
-
expressed in Escherichia coli
expressed in Escherichia coli and HeLa cells
-
expressed in Escherichia coli BL-21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)-RIL cells
-
expressed in Escherichia coli C41(DE3) cells
expressed in HEK-293 cells
-
expressed in HEK293A and MCF-7 cells
-
expression in H9C2 cell
-
expression in HEK-293A cells
-
expression in SH-SY5Y cells
-
human biliverdin reductase expressed in Escherichia coli
-
into pcDNA3 and expressed in 293A cells pcDNA3, into pGEX4-T2 and expressed in Escherichia coli
-
into the pGEM-T easy vector, subcloned into the vectors pcDNA3 and pESC-LEU
-
into the vector pcDNA3.1
into vector pcDNA3, transformed into Escherichia coli DH5alpha, pGEX-4T2/human BVR vector
-
kidney form of the enzyme cloned into the expression vector pGEX-KG
-
overexpressed in Escherichia coli as a His-tagged fusion protein
-
recombinant biliverdin reductase expressed in Escherichia coli
-
recombinant BVR is used
-
reversible overexpression of BVR in mouse fibroblasts, doxycycline-dependent overexpression of BVR in NIH 3T3 BVR-Tet-On cells confers protection against cytotoxic drugs cisplatin and doxorubicin
the expression of rat kidney biliverdin reductase is targeted by translational fusion of the chloroplast transit peptide sequence of the soybean small subunit of ribulose bisphosphate carboxylase gene to the enzyme cDNA, with this method transgenic Arabidopsis plants are cloned which express constitutive biliverdin reductase and display aberrant photomorphogenesis throughout their life-cycle
-
using the vector pBIB-KAN a plant transformation vector, containing the CAB3 promoter, a chloroplast targeting sequence and the BVR gene, is constructed, using pMON672 leads to a similar construct containing the MERI5 promoter