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1.3.1.32: maleylacetate reductase

This is an abbreviated version!
For detailed information about maleylacetate reductase, go to the full flat file.

Word Map on EC 1.3.1.32

Reaction

3-oxoadipate
+
NAD(P)+
=
2-maleylacetate
+
NAD(P)H
+
H+

Synonyms

GraC, hadD, hqdD, HqoD, HxqD, MAA reductase, maleoylacetate reductase, maleylacetate reductase, MAR, PcpE, pdcF, PnpD, TcpD, tfdF, tsdD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.32 maleylacetate reductase

Purification

Purification on EC 1.3.1.32 - maleylacetate reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme partially by anion exchange chromatography
native enzyme, over 246fold by several chromatographic steps
purified 433fold using DEAR-Toyopearl column chromatography, phenyl-sepharose column chromatography and Mono Q column chromatography
-
purified from a MacA overexpressing Escherichia coli clone
-
purified from induced 4-chlorophenol grown cells
-
purified from recombinant Escherichia coli cells
-
recombinant His-tagged wild-type and SeMet-labeled PnpD from Escherichia coli BL21 (DE3) by nickel affinity chromatography
recombinant poly-His tagged PcpE is purified to higher than 95% purity using affinity chromatography with a Ni-NTA agarose column