1.3.1.45: 2'-hydroxyisoflavone reductase

This is an abbreviated version!
For detailed information about 2'-hydroxyisoflavone reductase, go to the full flat file.

Reaction

Synonyms

CaIRL, GbIRL1, GmIFR, IFH, IFR, IRL, isoflavone 2'-hydroxylase, isoflavone reductase, isoflavone reductase-like protein, LOC100786578, More, NADPH-dependent isoflavone reductase, NADPH:2'-hydroxyisoflavone oxidoreductase, Ole e 12, OsIRL, PvIFR1, reductase, 2',7-dihydroxy-4',5'-methylenedioxyisoflavone, reductase, 2'-hydroxyisoflavone

ECTree

     1 Oxidoreductases

         1.3 Acting on the CH-CH group of donors

             1.3.1 With NAD⁺ or NADP⁺ as acceptor

                1.3.1.45 2'-hydroxyisoflavone reductase

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Results	in table
2	Activating Compound
122	AA Sequence
2	CAS Registry Number
12	Cloned(Commentary)
12	Cofactor
1	Crystallization (Commentary)
18	Expression
11	General Information
1	Inhibitors
9	KM Value [mM]
4	Localization
2	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
19	Organism
1	Oxidation Stability
5	Pathway
2	pH Optimum
2	pI Value
4	Posttranslational Modification
10	Protein Variants
7	Purification (Commentary)
1	Reaction
5	Reaction Type
18	Reference
32	Source Tissue
2	Specific Activity [micromol/min/mg]
2	Storage Stability
21	Substrates and Products (Substrate)
6	Subunits
36	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
7	Turnover Number [1/s]

Cloned

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Cloned on EC 1.3.1.45 - 2'-hydroxyisoflavone reductase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

cloning of full-length FcIRL, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, overview

Fagopyrum cymosum

A7Y232

713596

DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, transient expression of GFP-tagged enzyme in Glycine max protoplasts' cytoplasm, recombinant overexpression of the enzyme under the control of a CaMV35S promoter via Agrobacterium tumefaciens strain LBA4404 and tri-parental mating in transgenic Glycine max plants, quantitative real-time PCR enzyme expression analysis, recombinant expression of Nterminally His6-tagged enzyme in Escherichia coli strain Transetta (DE3)

Glycine max

C6TER4

744991

DNA and amino acid sequence dteremination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) by dialysis, nickel affinity chromatography, and proteolytic digestion of fusion protein with Factor Xa protease

Olea europaea

-

744406

expression in Escherichia coli

Pisum sativum

-

390815

expression in Nicotiana tabacum

Medicago sativa

-

390816

expression in transgenic Nicotiana tabacum plants under control of the CaMV35S promotor

Medicago sativa

-

656914

gene constructs lacking the promoter of CaIRL are introduced into Agrobacterium tumefaciens strain LBA4404 and used to transform tobacco leaf discs

Coffea arabica

C7BFZ4

700726

generating of transgenic rice lines overexpressing the OsIRL gene under an abscisic acid inducible promoter, the OsIRL transgenic rice line activated by abscisic acid treatment is tolerant against methyl viologen and glucose/glucose oxidase-induced stress in rice leaves and suspension-cultured cells, cloning of recombinant enzyme as six-His tagged OsIRL protein for antibody production

Oryza sativa

Q8VYH7

700650

into Escherichia coli expression vector pET28a

Medicago sativa

-

675379

isoflavone reductase-like gene gene by reverse transcription, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression in Escherichia coli strain BL21

Dendrobium catenatum

A0A068ERY8

745962

recombinantly expressed in Escherichia coli

Ginkgo biloba

M1T9X3

726152

sequence comparisons and phylogenetic tree, quantitative real-time PCR enzyme expression analysis

Phaseolus vulgaris

-

746131