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1.3.1.45: 2'-hydroxyisoflavone reductase

This is an abbreviated version!
For detailed information about 2'-hydroxyisoflavone reductase, go to the full flat file.

Word Map on EC 1.3.1.45

Reaction

vestitone
+
NADP+
=
2'-hydroxyformononetin
+
NADPH
+
H+

Synonyms

CaIRL, GbIRL1, GmIFR, IFH, IFR, IRL, isoflavone 2'-hydroxylase, isoflavone reductase, isoflavone reductase-like protein, LOC100786578, More, NADPH-dependent isoflavone reductase, NADPH:2'-hydroxyisoflavone oxidoreductase, Ole e 12, OsIRL, PvIFR1, reductase, 2',7-dihydroxy-4',5'-methylenedioxyisoflavone, reductase, 2'-hydroxyisoflavone

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.45 2'-hydroxyisoflavone reductase

Expression

Expression on EC 1.3.1.45 - 2'-hydroxyisoflavone reductase

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
After embedding of seeds in agar containing 2',7',-dichlorofluorescin, the H2O2 is highly stimulated in the elongation region compared with the root apex. The OsIRL mRNA expression level in 3-day-old roots is weakly expressed in the apex region, it shows an increment accompanying the increase in H2O2 concentration, results indicate that the expression level and localization of OsIRL is associated with H2O2 accumulation.
enzyme expression and transcript levels of PvIFR1, detected preferentially in the susceptible zone of roots, are augmented upon nitrogen starvation and in response to Rhizobium etli inoculation at very early stages of the interaction
-
higher enzyme expression in the leaf is recorded under the combined effect of heat stress (40/22 C day/night temperature for 4 days) and elevated CO2 (800 ppm)
-
increased expression of GbIRL1 is detected when the seedlings are treated with ultraviolet-B, 5-aminolevulinic acid, wounding and ethephon, abscisic acid, salicylic acid
OsIRL gene and protein are downregulated in young seedling roots treated with diphenyleneiodonium, known quencher of reactive oxygen species, but to a lesser extent when compared with glutathione treatment in roots
OsIRL gene and protein are downregulated in young seedling roots treated with reduced glutathione, a known quencher of reactive oxygen species, in roots of 3 day-old young seedlings grown on glutathione, reactive oxygen species levels are significantly decreased by approximately 50% in the presence of 1 mM glutathione compared with untreated controls
OsIRL gene and protein are shown to be downregulated in young seedling roots treated with reduced glutathione and diphenyleneiodonium
OsIRL transcript level in rice suspension-cultured cells is found to be by hydrogen peroxide, ferric chloride, methyl viologen and glucose/glucose oxidase
the enzyme expression is significantly induced by infection with Phytophthora sojae, as well as by treatment with ethephon, abscisic acid, and salicylic acid
the enzyme expression is upregulated in the common bacterial blight-resistant recombinant inbred line following Xanthomonas axonopodis treatment
-
the expression of OsIRL is slightly increased after treatment 0.02 mM abscisic acid in wild-type calli, suggesting a possible reason for glucose/glucose oxidase stress resistance
the full-length version of the CaIRL promoter directs a reporter gene expression that is stimulated by mechanical injury as the expression from the native CaIRL gene
the OsIRL transcript level in rice suspension-cultured cells is induced by FeCl3 within 4 h after treatment, but down-regulated when co-treated with glutathione
the OsIRL transcript level in rice suspension-cultured cells is induced by glucose/glucose oxidase within 4 h after treatment, but down-regulated when co-treated with glutathione
the OsIRL transcript level in rice suspension-cultured cells is induced by methyl viologen within 4 h after treatment, but down-regulated when co-treated with glutathione
the OsIRL transcript level in rice suspension-cultured cells is induced by the oxidant H2O2, but down-regulated when co-treated with glutathione
the promoter of a gene encoding an isoflavone reductase-like protein in coffee drives a stress-responsive expression in leaves
transcript level is markedly increased in response to fungal infection and mechanical injury, a rapid and marked increase in CaIRL expression is also observed following mechanical injury of the leaves (75fold after 4 h treatment), this induction is followed by a progressive decrease in transcript accumulation at 8 and 12 h post injury, the activation of CaIRL expression by mechanical injury is faster than that observed in response to fungal inoculation