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1.3.1.75: 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

This is an abbreviated version!
For detailed information about 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH), go to the full flat file.

Word Map on EC 1.3.1.75

Reaction

protochlorophyllide a
+
NADP+
=
3,8-divinyl protochlorophyllide a
 +
NADPH
 +
H+

Synonyms

3,8-divinyl (proto)chlorophyll(ide) a 8-vinyl reductase, 3,8-divinyl chlorophyllide reductase, 4VCR, 8-vinyl reductase, 8V reductase, 8VR, AM1_2394, AtDVR, AtN-DVR, BciA, BciA-type 8VR, bciB, C8 vinyl reductase, C8-vinyl reductase, C8V reductase, COR, CsDVR, CTBciA, ct_1063, cvrA, divinyl chlorophyll(ide) a reductase, divinyl chlorophyllide a 8-vinyl-reductase, divinyl chlorophyllide a 8-vinyl-reductase, chloroplastic, divinyl reductase, divinyl-chlorophyllide a reductase, DVR, N-DVR, NADPH-dependent 8VR, NADPH-dependent divinyl reductase, NADPH-dependent DVR, NADPH-dependent reductase BciA, nmrA, nucleoside-diphosphate-sugar epimerase, Os03g22780, OsDVR, PCB2, PhaeN-DVR, reductase, 4-vinylchlorophyllide a, RSBciA, RSP_3070, synw0963, ZmDVR, [3,8-divinyl]-protochlorophyllide a 8-vinyl reductase, [3,8-DV]-Chlide a 8-vinyl reductase, [4-vinyl]chlorophyllide a reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.75 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

Cloned

Cloned on EC 1.3.1.75 - 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli Rosetta2 cells
gene AM1_2394, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli
gene bchXYZa, cloning and DNA and amino acid sequence determination and analysis, the plasmid carrying genes bchXYZ is used for complementation fo bchXYZ-deficient mutant strains of Rhodobacter sphaeroides, Rhodobacter palustris, Chlorobium tepidum, and Rhodobacter castenholzii
-
gene bciA or nmrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, nmrA is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109
gene bciA or RCAP_rcc03260, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally Strep II-tagged enzyme in Escherichia coli strain Rosetta 2
gene bciA, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with the enzymes of the biosynthetic pathway, engineered pathway design for the heterologous production of bacteriochlorophyll in the non-photosynthetic host Escherichia coli, overview. RSBciA is active, albeit not in vivo with the engineered pathway. Subcloning in Escherichia coli strain JM109
gene bciA, recombinant expression in Escherichia coli, coexpression with the enzymes of the biosynthetic pathway, engineered pathway design for the heterologous production of bacteriochlorophyll in the non-photosynthetic host Escherichia coli, overview. CTBciA is capable of reducing the C8-vinyl group of several different intermediates in the BChl pathway. Subcloning in Escherichia coli strain JM109
gene DVR or PCB2, a single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons
gene DVR, a single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons
gene DVR, DNA and amino acid sequence determination and analysis, sequence comparisons
gene DVR, phylogenetic analysis
gene DVR, phylogenetic analysis, recombinant expression of NADPH-dependent DVR in Escherichia coli
-
gene synw0963, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli
Parasynechococcus marenigrum WH 8102
recombinant protein expressed in Escherichia coli