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1.3.1.9: enoyl-[acyl-carrier-protein] reductase (NADH)

This is an abbreviated version!
For detailed information about enoyl-[acyl-carrier-protein] reductase (NADH), go to the full flat file.

Word Map on EC 1.3.1.9

Reaction

an acyl-[acyl-carrier protein]
+
NAD+
=
a trans-2,3-dehydroacyl-[acyl-carrier protein]
 +
NADH
 +
H+

Synonyms

2-trans enoyl-ACP-reductase, 2-trans enoyl-acyl carrier protein reductase, 2-trans-enoyl-ACP (CoA) reductase, 2-trans-enoyl-ACP reductase, 2-trans-enoyl-ACP(CoA) reductase, ACP reductase, BaENR, BMA0885, bsFabI, cold-shock induced protein 15, CSI15, EACP reductase, enoyl (acyl carrier protein) reductase, enoyl ACP reductase, enoyl acyl carrier protein reductase, enoyl acyl carrier protein reductase InhA, enoyl reductase, enoyl-ACP reductase, enoyl-ACP reductase I, enoyl-ACP reductase III, enoyl-ACP(CoA) reductase, enoyl-acyl carrier protein, enoyl-acyl carrier protein reductase, enoyl-acyl carrier protein reductase I, enoyl-reductase, enoyl-[acyl-carrier-protein] reductase, ENR, ENR1, ENR2, FabI, FabI-1, FabI-related enoyl-ACP reductase, FabI1, FabI2, FabK, Fabl1, Fabl2, FabMG, FabV, FAS-II enoyl reductase, FTT_0782, InhA, More, MtENR, MtInhA, NAD-dependent enoyl-ACP reductase, NADH-dependent enoyl reductase, NADH-dependent enoyl-ACP reductase, NADH-dependent enoyl-acyl carrier protein reductase, NADH-enoyl acyl carrier protein reductase, NADH-ENR, NADH-specific enoyl-ACP reductase, OsmC, PA2950, pfENR, reductase, enoyl-[acyl carrier protein], trans-enoyl-[acyl carrier protein] reductase, trans-enoyl-[acyl-carrier-protein] reductase, VEG241, vegetative protein 241, VF_0888, YP_4011

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.9 enoyl-[acyl-carrier-protein] reductase (NADH)

Cloned

Cloned on EC 1.3.1.9 - enoyl-[acyl-carrier-protein] reductase (NADH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21 Star (DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Codon+-RIL cells
-
expressed in Escherichia coli BL21-Star(DE3) cells
-
expressed in Escherichia coli in BL-21(DE3) cells
-
expressed in Escherichia coli JP1111 cells
-
expressed in Escherichia coli Top10 cells
expressed with N-terminal hexa-histidine motifs in Escherichia coli BL21 (DE3) pLysS cells
expression in Escherichia coli
expression in Escherichia coli (BL21)
expression in Escherichia coli BL-21
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21(DE3)pLysS
expression of His-tagged InhA in Escherichia coli BL21(DE3)
expression of the His6-tagged ENR in Escherichia coli BL21
-
expression Saccharomyces cerevisiae etr1D cells lacking Etr1p, the 2-trans-enoyl-thioester reductase of mitochondrial type 2 fatty acid synthase. Yeast mitochondria are used as a surrogate compartment for hosting the drug-target protein InhA from mycobacterial FASII. The heterologous enzyme is ectopically expressed in a yeast mutant strain from which the native gene encoding the corresponding mitochondrial FASII enzyme is missing. Using an appropriate fungal mitochondrial leader sequence, the mycobacterial protein is directed to the mitochondria, where it can rescue the respiratory growth phenotype of the mutant. The rationale behind the assay is that added antimycolates are foreseen to inhibit the mycobacterial enzyme, thereby recreating the respiratory deficiency of the original mutant, discernible as poor colony formation and growth on glycerol medium
-
fabI gene from Escherichia coli DH5alpha was cloned into pBAD/Myc-His B vector for expression of the protein in Escherichia coli TOP10 cells
-
for expression in Escherichia coli BL21DE3 CodonPlus-RIL cells
-
gene fabI, overexpression in Escherichia coli strain M15
gene fabV, expression of His-tagged wild-type and mutant enzymes in Escherichia coli BL21(DE3), theN-terminal His-tag in bmFabv does not have a dramatic effect on catalytic activity
into a pET15b vector for expression in Escherichia coli BL21-Gold (DE3)
into the pET-21b+ vector for expression in the Escherichia coli methionine auxotroph B834DE3
into the pMALc2x vector without signal peptide coding sequence, a second construct contains in addition the sequence of the TEV protease cleaving site followed by a hexahistidine tag
-
overexpression in Escherichia coli
-
the enoyl-[acyl-carrier-protein] reductase gene of Escherichia coli is cloned into a vector coding for the 6 x His tag for expression in Escherichia coli BL21DE3 cells
the enoyl-[acyl-carrier-protein] reductase gene of Plasmodium falciparum is cloned into a vector coding for the 6 x His tag for expression in Escherichia coli BL21DE3 cells
-
the inhA gene was cloned into the pMK1 mycobacterial expression vector under the control of the strong promoter hsp60. The resulting construct is used to transform Mycobacterium bovis BCG Pasteur in order to allow over-production of recombinant His-tagged InhA