1.3.3.11: pyrroloquinoline-quinone synthase
This is an abbreviated version!
For detailed information about pyrroloquinoline-quinone synthase, go to the full flat file.
Word Map on EC 1.3.3.11
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1.3.3.11
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quinoproteins
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methanol
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prosthetic
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dehydrogenases
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radiodurans
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extorquens
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methylobacterium
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pqq-containing
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deinococcus
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calcoaceticus
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acinetobacter
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synthesis
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biotechnology
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analysis
- 1.3.3.11
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quinoproteins
- methanol
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prosthetic
- dehydrogenases
- radiodurans
- extorquens
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methylobacterium
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pqq-containing
- deinococcus
- calcoaceticus
-
acinetobacter
- synthesis
- biotechnology
- analysis
Reaction
Synonyms
PqqC, PqqC/D, pyrroloquinoline quinone synthase C, pyrroloquinoline-quinone synthase, pyrroloquinoline/quinone synthesis protein C, quinoprotein dehydrogenase
ECTree
1.3.3.11 pyrroloquinoline-quinone synthaseAdvanced search results
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results (Engineering
Engineering on EC 1.3.3.11 - pyrroloquinoline-quinone synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
H154S
site-directed mutagenesis, active site mutant, even with substrate PQQ bound, the enzyme is still in the open conformation with helices alpha5b and alpha6 unfolded and the active site solvent accessible, no product formation
R179S/Y175S
site-directed mutagenesis, active site mutant, the mutant shows an open conformation with a reaction intermediate trapped in the active site, the intermediate is tricyclic but nonplanar, implying that it has not undergone oxidatio, R179S/Y175S shows acceptable substrate complex crystals
Y175F
site-directed mutagenesis, active site mutant, the mutant shows a closed conformation indicating that Y175 is not required for the conformational change, no product formation
H154S
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site-directed mutagenesis, active site mutant, even with substrate PQQ bound, the enzyme is still in the open conformation with helices alpha5b and alpha6 unfolded and the active site solvent accessible, no product formation
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R179S/Y175S
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site-directed mutagenesis, active site mutant, the mutant shows an open conformation with a reaction intermediate trapped in the active site, the intermediate is tricyclic but nonplanar, implying that it has not undergone oxidatio, R179S/Y175S shows acceptable substrate complex crystals
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Y175F
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site-directed mutagenesis, active site mutant, the mutant shows a closed conformation indicating that Y175 is not required for the conformational change, no product formation
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additional information
additional information
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N60I mutation detected in PqqC deficiency mutant
additional information
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
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N60I mutation detected in PqqC deficiency mutant
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additional information
construction of a pqqC gene disruption mutant JX22MT1 by the EZ-Tn5 transposon mutation, the mutant shows no antifungal activity against Fusarium oxysporum f. sp. lycopersici in contrast to the wild-type strain JX22. Antifungal activity at wild-type level is restored by introduction of the functional wild-type pqqC gene
additional information
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construction of a pqqC gene disruption mutant JX22MT1 by the EZ-Tn5 transposon mutation, the mutant shows no antifungal activity against Fusarium oxysporum f. sp. lycopersici in contrast to the wild-type strain JX22. Antifungal activity at wild-type level is restored by introduction of the functional wild-type pqqC gene
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