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1.3.3.4: protoporphyrinogen oxidase

This is an abbreviated version!
For detailed information about protoporphyrinogen oxidase, go to the full flat file.

Word Map on EC 1.3.3.4

Reaction

protoporphyrinogen IX
+ 3 O2 =
protoporphyrin IX
+ 3 H2O2

Synonyms

HemG, HemG-type PPO, HemG-type protoporphyrinogen IX oxidase, hemY, hPPO, H_N10, H_N40, H_N90, LMJF_06_1280, mtPPO, MxPPOX, MxProtox, PPO, PPO1, ppo1-1, PPO2, PPOX, PPOX I, PPX1, PPX2, protein YfeX, protogen oxidase, protoporphyrinogen IX oxidase, protoporphyrinogen IX oxidase 1, protoporphyrinogen oxidase, protoporphyrinogen oxidase IX, protoporphyrinogenase, protox, Protox enzyme, R-PPO, Rs-slr1790 protein, S-PPO, Salk_143057, YfeX

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.4 protoporphyrinogen oxidase

Purification

Purification on EC 1.3.3.4 - protoporphyrinogen oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
both isozymes partially from cell culture of wild-type and transgenic cells, by chloroplast and mitochondria fractionation, detergent solubilization, and ultracentrifugation
-
by affinity chromatography
AY916795
by gel filtration
cloned enzyme
native enzyme from etioplasts and chloroplasts, recombinant enzyme from an Escherichia coli thioredoxin-deficient strain
-
Ni-IDA resin column chromatography
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography and Superdex 200 gel filtration
-
Ni2+ affinity column chromatography
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
Thermosynechococcus vestitus
-
recombinant His-tagged enzyme from Escherichia coli strain JM109 by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant V311M from Escherichia coli by nickel affinity chromatography
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by metal ion affinity chromatography
recombinant thioredoxin- and His-tagged, selenomethionine-labeled enzyme from Escherichia coli by nickel affinity chromatography, 3-step purification process
recombinant wild-type and mutant enzymes from Escherichia coli strain JM109, to homogeneity
-
recombinant wild-type and mutant enzymes from Escherichia coli strain XL-1 Blue inclusion bodies
to apparent homogeneity by Ni-IDA resin
-