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1.3.3.5: bilirubin oxidase

This is an abbreviated version!
For detailed information about bilirubin oxidase, go to the full flat file.

Word Map on EC 1.3.3.5

Reaction

2 bilirubin +

O2
= 2 biliverdin + 2 H2O

Synonyms

bilirubin oxidase, bilirubin oxidase M-1, bilirubin:oxygen oxidoreductase, blue Cu enzyme, BOD, BODx, BOX, BPUM_0542, copper oxidase, CotA, MCO, multicopper oxidase, MvBO, MvBOD, oxidase, bilirubin

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.5 bilirubin oxidase

Temperature Stability

Temperature Stability on EC 1.3.3.5 - bilirubin oxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
5 min, complete inactivation
20 - 30
-
at pH 2.7
20 - 60
20 - 70
-
at pH 9.0
22
-
pH 7.4, half-life: 25 h
4
-
pH 7.4, half-life: 300 h
40 - 50
-
the enzyme activity is stable after incubation for 1 h at a temperature up to 40°C, thermal stability is decreased beyond 50?C
40 - 61
-
sol-gel encapsulated BOD shows no decrease in relative activity until over 50°C while free enzyme loses some 40% activity at 40°C
40 - 90
at 40°C and 50°C, the enzyme retains 100% of its initial activity after 180 min of incubation. At 60°C, the enzyme shows approximately 100% of its maximum activity for 60 min. The enzyme has a half-life of 180 min at 70°C and 120 min at 80°C, respectively. At 90°C, the enzyme shows 85% of its initial activity after 30 min of incubation and the enzyme's half-life at this temperature is about 60 min. Moreover, the enzyme retains 39%, 29%, and 15% of its initial activity after incubation at 90°C for 90 min, 120 min, and 180 min, respectively
50
purified enzyme immobilized on a mesoporous carbon cryogel electrode displays high stability in solution at pH 5.0 and 50°C
50 - 70
-
the half life of BOD at 50°C is 114 min, residual activity after 30 min at 50°C is 83.3% of the original activity, at 70°C BOD activity disappears within several min
65
-
stable for 60 min
84
-
stable for 30 min