1.3.3.5: bilirubin oxidase
This is an abbreviated version!
For detailed information about bilirubin oxidase, go to the full flat file.
Word Map on EC 1.3.3.5
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1.3.3.5
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electrode
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oxidases
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biofuel
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cathode
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ceruloplasmin
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myrothecium
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anode
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laccases
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verrucaria
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biocathode
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ferroxidase
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trinuclear
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abts
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laccase-like
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dioxygen
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bioanode
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electrocatalytic
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bioelectrocatalytic
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2,6-dimethoxyphenol
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biliverdin
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cuprous
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multi-copper
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syringaldazine
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copa
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copper-binding
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four-electron
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mniii
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self-powered
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aceruloplasminemia
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hephaestin
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p-phenylenediamine
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manganeseii
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bioelectrodes
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open-circuit
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membrane-less
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cupredoxins
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analysis
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electrocatalysts
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manganese-oxidizing
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methionine-rich
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diazo
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biodevice
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energy production
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medicine
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diagnostics
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biotechnology
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synthesis
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environmental protection
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industry
- 1.3.3.5
-
electrode
- oxidases
-
biofuel
-
cathode
- ceruloplasmin
- myrothecium
-
anode
- laccases
- verrucaria
-
biocathode
- ferroxidase
-
trinuclear
- abts
-
laccase-like
- dioxygen
-
bioanode
-
electrocatalytic
-
bioelectrocatalytic
- 2,6-dimethoxyphenol
- biliverdin
-
cuprous
-
multi-copper
- syringaldazine
- copa
-
copper-binding
-
four-electron
-
mniii
-
self-powered
-
aceruloplasminemia
- hephaestin
- p-phenylenediamine
-
manganeseii
-
bioelectrodes
-
open-circuit
-
membrane-less
- cupredoxins
- analysis
-
electrocatalysts
-
manganese-oxidizing
-
methionine-rich
-
diazo
-
biodevice
- energy production
- medicine
- diagnostics
- biotechnology
- synthesis
- environmental protection
- industry
Reaction
2 bilirubin + = 2 biliverdin + 2 H2O
Synonyms
bilirubin oxidase, bilirubin oxidase M-1, bilirubin:oxygen oxidoreductase, blue Cu enzyme, BOD, BODx, BOX, BPUM_0542, copper oxidase, CotA, MCO, multicopper oxidase, MvBO, MvBOD, oxidase, bilirubin
ECTree
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Temperature Stability
Temperature Stability on EC 1.3.3.5 - bilirubin oxidase
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20 - 60
37
40 - 50
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the enzyme activity is stable after incubation for 1 h at a temperature up to 40°C, thermal stability is decreased beyond 50?C
40 - 61
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sol-gel encapsulated BOD shows no decrease in relative activity until over 50°C while free enzyme loses some 40% activity at 40°C
40 - 90
at 40°C and 50°C, the enzyme retains 100% of its initial activity after 180 min of incubation. At 60°C, the enzyme shows approximately 100% of its maximum activity for 60 min. The enzyme has a half-life of 180 min at 70°C and 120 min at 80°C, respectively. At 90°C, the enzyme shows 85% of its initial activity after 30 min of incubation and the enzyme's half-life at this temperature is about 60 min. Moreover, the enzyme retains 39%, 29%, and 15% of its initial activity after incubation at 90°C for 90 min, 120 min, and 180 min, respectively
50
purified enzyme immobilized on a mesoporous carbon cryogel electrode displays high stability in solution at pH 5.0 and 50°C
50 - 70
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the half life of BOD at 50°C is 114 min, residual activity after 30 min at 50°C is 83.3% of the original activity, at 70°C BOD activity disappears within several min
60
70
80
20 - 60
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1 h incubation at 60°C, free enzyme retains 26% of its original activity, immobilized enzyme 73%
37
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soluble enzyme, half-life: 12 h immobilized enzyme, half-life: 63 h
37
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purified enzyme, pH 7.0, no activity decrease after preincubation for 300 min
37
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purified recombinant enzyme, half-life for deactivation is more than 300 min
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half-life of the recombinant enzyme expressed in Escherichia coli is 90 min, half-life of authentic enzyme is 15 min
60
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irreversible thermal inactivation, dynamics and kinetics, overview
60
half-life of the wild-type enzyme is 15 min, and of the recombinant enzyme 90 min
60
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purified recombinant enzyme, half-life for deactivation is about 70 min
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lyophilized enzyme stored at 70°C in the presence of polyvinylalcohol for 5 h, 40% of acitivity are lost after rehydration
70
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lyophilized enzyme stored at 70°C in the presence of alpha,beta-poly(N-hydroxyethyl)-L-aspartamide for 5 h, about 10% of acitivity are lost after rehydration
80
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purified enzyme, pH 7.0, less than 50% loss in activity after preincubation for 90 min