Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
11000
-
1 * 66000 + 1 * 27000 + 1 * 12000 + 1 * 11000, SDS-PAGE
110000 - 114000
-
excluding cytochrome b, calculation from FAD-content and subunit composition
118000
-
calculation from subunit composition
119800
-
monomeric recombinant C-terminally His6-tagged enzyme, gel filtration
12800
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
135000
-
high resolution clear-native electrophoresis
13900
-
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900
14080
x * 63075 (flavoprotein subunit SdhA) + x * 36471 (iron-sulfur protein SdhB) + x * 32205 (subunit SdhC) + x * 14080 (subunit SdhD). Subunit SdhA and SdhB show characteristic sequence similarities to the succinate dehydrogenases and fumarate reductases of other organisms, while the SdhC and SdhD subunits, thought to form the membrane-anchoring domain, lack typical transmembrane alpha-helical regions present in all other succinate:quinone reductases and quinol:fumarate reductases
14300
-
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900
147000
-
calculation from Stokes radius and sedimentation coefficient
150000
-
blue native gel electrophoresis
160000 - 167000
-
including cytochrome b, calculation from FAD-content and subunit composition
16638
-
1 * 70185, + 1 * 30229, + 1 * 16675, + 1 * 16638, subunits Sdh1, Sdh2, Sdh3 and Sdh4
16675
-
1 * 70185, + 1 * 30229, + 1 * 16675, + 1 * 16638, subunits Sdh1, Sdh2, Sdh3 and Sdh4
17000
-
1 * 72000 (flavoprotein) + 1* 30000, and 1 * 15000 + 1 * 17000 as membrane anchor (at least one of the latter a cytochrome b-protein), precipitation of protein with specific antibody and SDS-PAGE
170000
-
sedimentation equilibrium centrifugation of preparation containing Triton X-100
18000
-
1 * 70000, flavoprotein, + 1 * 32000, iron-sulfur protein, + 1 * 18000, SDS-PAGE
180000
-
dimeric form, gel filtration
23000
-
3 * 29000 + 3 * 67000 + 3 * 23000, homotrimeric complex of the protomer composed of three different subunits (29000 Da, 67000 Da and 23000 Da), SDS-PAGE
260000
-
Blue-native PAGE
29000
-
3 * 29000 + 3 * 67000 + 3 * 23000, homotrimeric complex of the protomer composed of three different subunits (29000 Da, 67000 Da and 23000 Da), SDS-PAGE
29600
-
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900
30229
-
1 * 70185, + 1 * 30229, + 1 * 16675, + 1 * 16638, subunits Sdh1, Sdh2, Sdh3 and Sdh4
32205
x * 63075 (flavoprotein subunit SdhA) + x * 36471 (iron-sulfur protein SdhB) + x * 32205 (subunit SdhC) + x * 14080 (subunit SdhD). Subunit SdhA and SdhB show characteristic sequence similarities to the succinate dehydrogenases and fumarate reductases of other organisms, while the SdhC and SdhD subunits, thought to form the membrane-anchoring domain, lack typical transmembrane alpha-helical regions present in all other succinate:quinone reductases and quinol:fumarate reductases
35000
-
iron-sulphur subunit
36471
x * 63075 (flavoprotein subunit SdhA) + x * 36471 (iron-sulfur protein SdhB) + x * 32205 (subunit SdhC) + x * 14080 (subunit SdhD). Subunit SdhA and SdhB show characteristic sequence similarities to the succinate dehydrogenases and fumarate reductases of other organisms, while the SdhC and SdhD subunits, thought to form the membrane-anchoring domain, lack typical transmembrane alpha-helical regions present in all other succinate:quinone reductases and quinol:fumarate reductases
37000
-
x * 66000 + x * 37000 + x * 33000 + x * 12000, presence of very strong protein protein interactions among the 66000 Da, the 37000 Da and the 12000 Da subunit, and of weaker interactions between the 33000 Da subunit and the rest of the subunits, SDS-PAGE
54000
-
the enzyme forms a trimer of single subunits consisting of four polypeptides: 1 * 54000, flavoprotein SdhA, + 1 * 27000, iron-sulfur protein SdhB, + 1 * 14000, SdhC, + 1 * 15000, membrane anchor protein SdhD, SDS-PAGE
60000
-
1 * 60000 + 1* 25000, SDS-PAGE
62000
-
? * 62000 + ? * 26000
63000
x * 63000, SDS-PAGE
63075
x * 63075 (flavoprotein subunit SdhA) + x * 36471 (iron-sulfur protein SdhB) + x * 32205 (subunit SdhC) + x * 14080 (subunit SdhD). Subunit SdhA and SdhB show characteristic sequence similarities to the succinate dehydrogenases and fumarate reductases of other organisms, while the SdhC and SdhD subunits, thought to form the membrane-anchoring domain, lack typical transmembrane alpha-helical regions present in all other succinate:quinone reductases and quinol:fumarate reductases
64000
-
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE
65600
-
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900
69000
-
fumarate reductase, membrane-extrinsic domain: 1 * 69000 + 1 * 27000, membrane-intrinsic domain: 1 * 15000 + 1 * 13000 containing cytochrome b, necessary for converting succinate dehydrogenase EC 1.3.99.1 into succinate-ubiquinone oxidoreductase
7000
-
1 * 7000 + 1* 27000, SDS-PAGE
70185
-
1 * 70185, + 1 * 30229, + 1 * 16675, + 1 * 16638, subunits Sdh1, Sdh2, Sdh3 and Sdh4
71000
-
succinate dehydrogenase, 1 * 71000 + 1 * 26000 + 1 + 17000 + 1 * 15000, immunoprecipitation followed by SDS-PAGE
73000
-
1 * 73000 + 1 * 27000 + 1 * 30000, three structures of the enzyme based on three different crystal forms are available, in all three crystal forms two heterotrimeric complexes of A,B and C subunits are associated in an identical fashion, forming a dimer
100000
-
gel filtration
100000
-
succinate dehydrogenase
100000
-
SDS-PAGE after cross-linkage with dimethylsuberimidate, sedimentation equilibrium centrifugation
11100
-
x * 68000 (subunit 1) + x * 28400 (subunit 2) + x * 15300 (subunit 3) + x * 11100 (subunit 4), calculated from sequence
11100
-
x * 68000 (subunit 1) + x * 28400 (subunit 2) + x * 15300 (subunit 3) + x * 11100 (subunit 4), calculated from sequence
12000
-
1 * 66000 + 1 * 27000 + 1 * 12000 + 1 * 11000, SDS-PAGE
12000
-
x * 66000 + x * 37000 + x * 33000 + x * 12000, presence of very strong protein protein interactions among the 66000 Da, the 37000 Da and the 12000 Da subunit, and of weaker interactions between the 33000 Da subunit and the rest of the subunits, SDS-PAGE
120000
-
-
120000
-
larval and adult complex II, non-denaturating PAGE
120000
3 * 120000 Da, SQR is packed as a trimer, determined by crystal structure analysis
120000
-
1 * 120000, about, recombinant C-terminally His6-tagged enzyme, SDS-PAGE, trimerization is disrupted in rcIISdhB-His6 due to the insertion of a hexahistidine tag on the C-terminus of SdhB subunit and the resulting protein complex can only form a monomer
120000
-
3 * 120000, about, wild-type enzyme, SDS-PAGE
120000
-
4 * 120000, about, recombinant N-terminally His8-tagged enzyme, SDS-PAGE, four subunits of the rcII-His8-SdhB complex
13000
-
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE
13000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
13000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
13000
-
fumarate reductase, membrane-extrinsic domain: 1 * 69000 + 1 * 27000, membrane-intrinsic domain: 1 * 15000 + 1 * 13000 containing cytochrome b, necessary for converting succinate dehydrogenase EC 1.3.99.1 into succinate-ubiquinone oxidoreductase
14000
-
1 * 72000, 1 * 28000, 1 * 14000
14000
-
the enzyme forms a trimer of single subunits consisting of four polypeptides: 1 * 54000, flavoprotein SdhA, + 1 * 27000, iron-sulfur protein SdhB, + 1 * 14000, SdhC, + 1 * 15000, membrane anchor protein SdhD, SDS-PAGE
14000
x * 67000 + x * 33000 + x * 28000 + x * 14000, SDS-PAGE
15000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
15000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
15000
-
succinate dehydrogenase, 1 * 71000 + 1 * 26000 + 1 + 17000 + 1 * 15000, immunoprecipitation followed by SDS-PAGE
15000
-
fumarate reductase, membrane-extrinsic domain: 1 * 69000 + 1 * 27000, membrane-intrinsic domain: 1 * 15000 + 1 * 13000 containing cytochrome b, necessary for converting succinate dehydrogenase EC 1.3.99.1 into succinate-ubiquinone oxidoreductase
15000
-
1 * 72000 (flavoprotein) + 1* 30000, and 1 * 15000 + 1 * 17000 as membrane anchor (at least one of the latter a cytochrome b-protein), precipitation of protein with specific antibody and SDS-PAGE
15000
-
the enzyme forms a trimer of single subunits consisting of four polypeptides: 1 * 54000, flavoprotein SdhA, + 1 * 27000, iron-sulfur protein SdhB, + 1 * 14000, SdhC, + 1 * 15000, membrane anchor protein SdhD, SDS-PAGE
15300
-
x * 68000 (subunit 1) + x * 28400 (subunit 2) + x * 15300 (subunit 3) + x * 11100 (subunit 4), calculated from sequence
15300
-
x * 68000 (subunit 1) + x * 28400 (subunit 2) + x * 15300 (subunit 3) + x * 11100 (subunit 4), calculated from sequence
200000
-
including cytochrome b, sedimentation equilibrium centrifugation
200000
-
calculated from the molar mass of the enzyme particle and its contents of Triton and phospholipid
25000
-
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE
25000
-
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000
25000
-
1 * 79000 + 1 * 31000 + 1 * 25000
26000
-
succinate dehydrogenase, 1 * 71000 + 1 * 26000 + 1 + 17000 + 1 * 15000, immunoprecipitation followed by SDS-PAGE
26000
-
1 * 65000 + 1 * 26000, large subunit is a flavoprotein, small subunit is an iron-sulfur protein
26000
-
? * 62000 + ? * 26000
27000
-
1 * 66000 + 1 * 27000 + 1 * 12000 + 1 * 11000, SDS-PAGE
27000
-
fumarate reductase, membrane-extrinsic domain: 1 * 69000 + 1 * 27000, membrane-intrinsic domain: 1 * 15000 + 1 * 13000 containing cytochrome b, necessary for converting succinate dehydrogenase EC 1.3.99.1 into succinate-ubiquinone oxidoreductase
27000
-
1 * 73000 + 1 * 27000 + 1 * 30000, three structures of the enzyme based on three different crystal forms are available, in all three crystal forms two heterotrimeric complexes of A,B and C subunits are associated in an identical fashion, forming a dimer
27000
-
the enzyme forms a trimer of single subunits consisting of four polypeptides: 1 * 54000, flavoprotein SdhA, + 1 * 27000, iron-sulfur protein SdhB, + 1 * 14000, SdhC, + 1 * 15000, membrane anchor protein SdhD, SDS-PAGE
27097
-
1 * 73234 + 1 * 28064 + 1 * 27097 Da calculated for flavoprotein, iron-sulfur protein, and cytochrome subunit, respectively. Complex is composed of three subunits, a 74 kDa flavoprotein that contains a covalently bound flavin adenine dinucleotide, a 28 kDa iron-sulfur cluster-containing polypeptide, and a 27 kDa transmembrane polypeptide, which is also the binding site of two b-type hemes and two menaquinones
27097
-
1 * 73234 + 1 * 28064 + 1 * 27097 Da calculated for flavoprotein, iron-sulfur protein, and cytochrome subunit. Complex is composed of three subunits, a 74 kDa flavoprotein that contains a covalently bound flavin adenine dinucleotide, a 28 kDa iron-sulfur cluster-containing polypeptide, and a 27 kDa transmembrane polypeptide, which is also the binding site of two b-type hemes and two menaquinones
28000
-
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE
28000
-
1 * 72000, 1 * 28000, 1 * 14000
28000
-
1 * 65000 (flavoprotein) + 1 * 28000 (ironprotein) + 19000 (cytochrome protein, membrane anchor), precipitation of radiolabeled protein with specific antibody and SDS-PAGE
28000
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
28000
x * 67000 + x * 33000 + x * 28000 + x * 14000, SDS-PAGE
28064
-
1 * 73234 + 1 * 28064 + 1 * 27097 Da calculated for flavoprotein, iron-sulfur protein, and cytochrome subunit, respectively. Complex is composed of three subunits, a 74 kDa flavoprotein that contains a covalently bound flavin adenine dinucleotide, a 28 kDa iron-sulfur cluster-containing polypeptide, and a 27 kDa transmembrane polypeptide, which is also the binding site of two b-type hemes and two menaquinones
28064
-
1 * 73234 + 1 * 28064 + 1 * 27097 Da calculated for flavoprotein, iron-sulfur protein, and cytochrome subunit. Complex is composed of three subunits, a 74 kDa flavoprotein that contains a covalently bound flavin adenine dinucleotide, a 28 kDa iron-sulfur cluster-containing polypeptide, and a 27 kDa transmembrane polypeptide, which is also the binding site of two b-type hemes and two menaquinones
28400
-
x * 68000 (subunit 1) + x * 28400 (subunit 2) + x * 15300 (subunit 3) + x * 11100 (subunit 4), calculated from sequence
28400
-
x * 68000 (subunit 1) + x * 28400 (subunit 2) + x * 15300 (subunit 3) + x * 11100 (subunit 4), calculated from sequence
30000
-
sum of molecular weights of membrane anchor subunits is about 30 kDa
30000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
30000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
30000
-
1 * 73000 + 1 * 27000 + 1 * 30000, three structures of the enzyme based on three different crystal forms are available, in all three crystal forms two heterotrimeric complexes of A,B and C subunits are associated in an identical fashion, forming a dimer
31000
-
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE
31000
-
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000
31000
-
1 * 79000 + 1 * 31000 + 1 * 25000
31000
-
1 * 79000 + 1 * 31000, SDS-PAGE
31000
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
32000
-
x * 32000, recombinant SDISP, SDS-PAGE
32000
-
1 * 70000, flavoprotein, + 1 * 32000, iron-sulfur protein, + 1 * 18000, SDS-PAGE
33000
-
x * 66000 + x * 37000 + x * 33000 + x * 12000, presence of very strong protein protein interactions among the 66000 Da, the 37000 Da and the 12000 Da subunit, and of weaker interactions between the 33000 Da subunit and the rest of the subunits, SDS-PAGE
33000
x * 67000 + x * 33000 + x * 28000 + x * 14000, SDS-PAGE
410000
-
PAGE
410000
-
non-denaturing PAGE
500000
-
native PAGE
500000
-
wild-type enzyme, consisting of 360 kDa from protein and an unknown contribution of detergent and lipid, native PAGE
65000
-
1 * 65000 + 1 * 26000, large subunit is a flavoprotein, small subunit is an iron-sulfur protein
65000
-
1 * 65000 (flavoprotein) + 1 * 28000 (ironprotein) + 19000 (cytochrome protein, membrane anchor), precipitation of radiolabeled protein with specific antibody and SDS-PAGE
66000
-
1 * 66000 + 1 * 27000 + 1 * 12000 + 1 * 11000, SDS-PAGE
66000
-
x * 70000, about, HA-tagged Sdh1p, SDS-PAGE, x * 66000, cleaved HA-tagged Sdh1p, SDS-PAGE
66000
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
66000
-
x * 66000 + x * 37000 + x * 33000 + x * 12000, presence of very strong protein protein interactions among the 66000 Da, the 37000 Da and the 12000 Da subunit, and of weaker interactions between the 33000 Da subunit and the rest of the subunits, SDS-PAGE
67000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
67000
-
1 * 67000 + 1 * 30000 + 1 * 15000 + 1 * 13000, SDS-PAGE
67000
-
3 * 29000 + 3 * 67000 + 3 * 23000, homotrimeric complex of the protomer composed of three different subunits (29000 Da, 67000 Da and 23000 Da), SDS-PAGE
67000
x * 67000 + x * 33000 + x * 28000 + x * 14000, SDS-PAGE
68000
-
x * 68000 (subunit 1) + x * 28400 (subunit 2) + x * 15300 (subunit 3) + x * 11100 (subunit 4), calculated from sequence
68000
-
x * 68000 (subunit 1) + x * 28400 (subunit 2) + x * 15300 (subunit 3) + x * 11100 (subunit 4), calculated from sequence
70000
-
flavoprotein subunit, identified by Western blot analysis
70000
-
x * 70000, about, HA-tagged Sdh1p, SDS-PAGE, x * 66000, cleaved HA-tagged Sdh1p, SDS-PAGE
70000
x * 70000, flavoprotein of succinate dehydrogenase, SDS-PAGE
70000
-
1 * 70000, flavoprotein, + 1 * 32000, iron-sulfur protein, + 1 * 18000, SDS-PAGE
72000
-
1 * 72000, 1 * 28000, 1 * 14000
72000
-
1 * 72000 (flavoprotein) + 1* 30000, and 1 * 15000 + 1 * 17000 as membrane anchor (at least one of the latter a cytochrome b-protein), precipitation of protein with specific antibody and SDS-PAGE
73234
-
1 * 73234 + 1 * 28064 + 1 * 27097 Da calculated for flavoprotein, iron-sulfur protein, and cytochrome subunit, respectively. Complex is composed of three subunits, a 74 kDa flavoprotein that contains a covalently bound flavin adenine dinucleotide, a 28 kDa iron-sulfur cluster-containing polypeptide, and a 27 kDa transmembrane polypeptide, which is also the binding site of two b-type hemes and two menaquinones
73234
-
1 * 73234 + 1 * 28064 + 1 * 27097 Da calculated for flavoprotein, iron-sulfur protein, and cytochrome subunit. Complex is composed of three subunits, a 74 kDa flavoprotein that contains a covalently bound flavin adenine dinucleotide, a 28 kDa iron-sulfur cluster-containing polypeptide, and a 27 kDa transmembrane polypeptide, which is also the binding site of two b-type hemes and two menaquinones
79000
-
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE
79000
-
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000
79000
-
1 * 79000 + 1 * 31000 + 1 * 25000
79000
-
1 * 79000 + 1 * 31000, SDS-PAGE
90000 - 100000
-
monomeric form, gel filtration
90000 - 100000
-
soluble enzyme, gel filtration, ultracentrifugation
97000 - 105000
-
sedimentation
97000 - 105000
-
heart, sedimentation, chromatography on agarose, calculation from FAD content