Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.3.7.15: chlorophyllide a reductase

This is an abbreviated version!
For detailed information about chlorophyllide a reductase, go to the full flat file.

Word Map on EC 1.3.7.15

Reaction

3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a
+ 2 oxidized ferredoxin [iron-sulfur] cluster +
ADP
 +
phosphate
=
3-devinyl-3-(1-hydroxyethyl)chlorophyllide a
 + 2 reduced ferredoxin [iron-sulfur] cluster +
ATP
 +
H2O
 + 2 H+

Synonyms

(BchY/BchZ)2 protein, a-COR, BchA, bchB, bchL, bchN, BchX, BchX/Y/Z, BchXYZ, BchY, BchZ, chlorophyllide a oxidoreductase, chlorophyllide oxidoreductase, chlorophyllide reductase, COR, COR 8VR, dark-operative protochlorophyllide oxidoreductase, DPOR, EC 1.3.99.35, light-independent chlorophyllide reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.7 With an iron-sulfur protein as acceptor
                1.3.7.15 chlorophyllide a reductase

Purification

Purification on EC 1.3.7.15 - chlorophyllide a reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
copurification of recombinant GST-tagged BchX and BchY from Escherichia coli by glutathione affinity chromatography
Ni2+-loaded chelating Sepharose column chromatography
-
recombinant N-terminally His6-tagged BchY and untagged BchZ as a subcomplex, and N-terminally thioredoxin/His/S-tagged BchX2, from Escherichia coli strain BL21(DE3) by nickel affinity chromatography under strict anaerobic conditions (i.e. in an anaerobic chamber), which is essential for the preservation of enzymatic activities. Method evaluation, overview
P26277; Q16DU8; Q16DU7
Strep-Tactin, column chromatography
-