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1.3.7.3: phycoerythrobilin:ferredoxin oxidoreductase

This is an abbreviated version!
For detailed information about phycoerythrobilin:ferredoxin oxidoreductase, go to the full flat file.

Word Map on EC 1.3.7.3

Reaction

(3Z)-phycoerythrobilin
+
oxidized ferredoxin
=
15,16-dihydrobiliverdin
+
reduced ferredoxin
+ 2 H+

Synonyms

bilin reductase, ferredoxin:3Z-phycoerythrobilin oxidoreductase, GtPEBB, oxidoreductase, ferredoxin:3Z-phycoerythrobilin, PEB:ferredoxin oxidoreductase, PebB

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.7 With an iron-sulfur protein as acceptor
                1.3.7.3 phycoerythrobilin:ferredoxin oxidoreductase

Cloned

Cloned on EC 1.3.7.3 - phycoerythrobilin:ferredoxin oxidoreductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed using a tac promoter-driven N-terminal GST fusion protein
-
expression in Escherichia coli
-
gene pebB, in Synechococcus sp. WH8020, the genes encoding for pebA and pebB share an overlapping region. The pebA stop codon TGA is part of the pebB start codon ATG, recombinant expression of a translational fusion between pebA and pebB, N-terminally Strep-tagged and C-terminally His-tagged PebAgB, recombinant expression of GST-tagged or His-tagged PebB in Escherichia coli BL21(DE3)
gene pebB, recombinant expression of the codon optimized gene, without signal peptide, in Escherichia coli, expression of GST-tagged wild-type and mutant enzymes in Escherichia coli
gene pebB, recombinant expression of wild-type and mutant enzymes
-
into pASK-IBA45+ and transformed in Escherichia coli DH10B cells
into vector pKT270, co-expression with cyanobacterial phytochrome 1 in Escherichia coli JM109
-