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1.3.7.3: phycoerythrobilin:ferredoxin oxidoreductase

This is an abbreviated version!
For detailed information about phycoerythrobilin:ferredoxin oxidoreductase, go to the full flat file.

Word Map on EC 1.3.7.3

Reaction

(3Z)-phycoerythrobilin
+
oxidized ferredoxin
=
15,16-dihydrobiliverdin
 +
reduced ferredoxin
 + 2 H+

Synonyms

bilin reductase, ferredoxin:3Z-phycoerythrobilin oxidoreductase, GtPEBB, oxidoreductase, ferredoxin:3Z-phycoerythrobilin, PEB:ferredoxin oxidoreductase, PebB

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.7 With an iron-sulfur protein as acceptor
                1.3.7.3 phycoerythrobilin:ferredoxin oxidoreductase

Purification

Purification on EC 1.3.7.3 - phycoerythrobilin:ferredoxin oxidoreductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by two affinity chromatography steps to 90% purity and a third gel filtration step
-
GST-tagged PebA forms inactive aggregates
-
recombinant GST-tagged or His-tagged PebB from Escherichia coli BL21(DE3) by glutathione or nickel affinity chromatography, gel filtration, and ultrafiltration, recombinant tagged PebAgB by affinity chromatography
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli by DNA removal from cell extract, ultracentrifugation at 220000 x g, glutathione affinity chromatography and cleavage of the tag through TEV protease, followed by dialysis and a second step of glutathione affinity chromatography to remove that tag, gel filtration, and ultrafiltration