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2-amino-benzoyl-CoA + reduced methyl viologen + ATP
2-amino-cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
at 11% of the velocity of benzoyl-CoA
-
-
?
2-aminobenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 18% of the activity relative to benzoyl-CoA
-
-
?
2-bromobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-bromocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
2-chloro-benzoyl-CoA + reduced methyl viologen + ATP
2-chloro-cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
at 106% of the velocity of benzoyl-CoA
-
-
?
2-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
2-fluoro-benzoyl-CoA + reduced methyl viologen + ATP
2-fluoro-cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
at 145% of the velocity of benzoyl-CoA
-
-
?
2-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP
?
2-hydroxy-benzoyl-CoA + reduced methyl viologen + ATP
2-hydroxy-cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
at 64% of the velocity of benzoyl-CoA
-
-
?
2-hydroxybenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
2-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
3-bromobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-bromocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
3-fluoro-benzoyl-CoA + reduced methyl viologen + ATP
3-fluoro-cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
at 31% of the velocity of benzoyl-CoA
-
-
?
3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
3-fluorobenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 23% of the activity relative to benzoyl-CoA
-
-
?
3-hydroxybenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
3-hydroxybenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 12% of the activity relative to benzoyl-CoA
-
-
?
3-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
3-methylbenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 12% of the activity relative to benzoyl-CoA
-
-
?
4-bromobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
4-bromocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
4-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
4-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
4-ethylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
4-ethylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
4-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
4-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
4-hydroxybenzoyl-CoA + reduced methyl viologen + ATP
4-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized methyl viologen + ADP + phosphate
-
at 5% of the activity relative to benzoyl-CoA
-
-
?
4-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
4-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
azide + ATP + H2O
?
-
-
-
-
?
Benzoyl-CoA + reduced acceptor + ATP
?
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
benzoyl-CoA + reduced ferredoxin + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + ferredoxin + ADP + phosphate
-
-
-
-
?
benzoyl-CoA + reduced ferredoxin + ATP + H2O
cyclohexa-1,5-diene-1-carboxyl-CoA + oxidized ferredoxin + ADP + phosphate
-
-
-
-
?
benzoyl-CoA + reduced methyl viologen + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
-
-
-
?
furan-2-carbonyl-CoA + reduced methyl viologen + ATP
? + methyl viologen + ADP + phosphate
-
at 28% of the velocity of benzoyl-CoA
-
-
?
N-methylhydroxylamine + ATP + H2O
?
-
-
-
-
?
O-methylhydroxylamine + ATP + H2O
?
-
-
-
-
?
pyridine-2-carbonyl-CoA + reduced methyl viologen + ATP
? + methyl viologen + ADP + phosphate
-
at 67% of the velocity of benzoyl-CoA
-
-
?
pyridine-4-carbonyl-CoA + reduced methyl viologen + ATP
? + methyl viologen + ADP + phosphate
-
at 41% of the velocity of benzoyl-CoA
-
-
?
thiophene-2-carbonyl-CoA + reduced methyl viologen + ATP
? + methyl viologen + ADP + phosphate
-
at 144% of the velocity of benzoyl-CoA
-
-
?
thiophene-3-carbonyl-CoA + reduced methyl viologen + ATP
? + methyl viologen + ADP + phosphate
-
at 10% of the velocity of benzoyl-CoA
-
-
?
additional information
?
-
2-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
low activity
-
-
?
2-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
2-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
2-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
2-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
high activity
-
-
?
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 78% of the activity relative to benzoyl-CoA
-
-
?
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 10% of the activity relative to benzoyl-CoA
-
-
?
2-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
best substrate of enzyme BCRTar
-
-
?
2-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-bromobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-bromocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
3-bromobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-bromocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-bromobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-bromocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
high activity
-
-
?
3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
3-fluorobenzoyl-CoA is converted only to the fluorinated cyclic dienoyl-CoA compound
-
-
?
3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
3-hydroxybenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
low activity
-
-
?
3-hydroxybenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-hydroxybenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
high activity
-
-
?
3-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
best substrate of enzyme MBRTcl
-
-
?
4-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
4-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
low activity
-
-
?
4-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
4-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
Benzoyl-CoA + reduced acceptor + ATP
?
-
induced by benzoyl-CoA
-
-
?
Benzoyl-CoA + reduced acceptor + ATP
?
-
key enzyme of anaerobic aromatic metabolism
-
-
?
Benzoyl-CoA + reduced acceptor + ATP
?
-
key enzyme of anaerobic aromatic metabolism
-
-
?
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
-
-
?
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
-
-
?
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
-
-
ir
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
acceptor: Ti3+, reduced methyl viologen
-
?
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
-
-
ir
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
-
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
the enzyme is involved in the anaerobic catabolism of benzoate
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
rate-limiting enzyme of anaerobic benzoate degradation. badM encodes a transcriptional repressor of benzoyl-CoA reductase gene expression. BadM controls gene expression from the benzoyl-CoA reductase promoter in concert with two transcriptional activators
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
-
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
the enzyme is involved in the anaerobic catabolism of benzoate
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
central enzyme in the anaerobic degradation of organic carbon
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
high activity
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
high activity
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
high activity
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
substrate binding structure analysis
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
substrate binding structure analysis
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
substrate binding structure analysis
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
substrate binding structure analysis
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
high activity
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
high activity
-
-
?
NH2OH + ATP + H2O
?
-
-
-
-
?
NH2OH + ATP + H2O
?
-
-
-
-
?
additional information
?
-
-
class II BCR from Desulfosarcina cetonica slowly disproportionates two 1,5-dienoyl-CoA to BzCoA and 1-monenoyl-CoA at about 0.1% of the 1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity rate. Assays for BzCoA reduction are perfomred by flavin-based electron bifurcation
-
-
-
additional information
?
-
-
class II BCR from Desulfosarcina cetonica slowly disproportionates two 1,5-dienoyl-CoA to BzCoA and 1-monenoyl-CoA at about 0.1% of the 1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity rate. Assays for BzCoA reduction are perfomred by flavin-based electron bifurcation
-
-
-
additional information
?
-
-
class II BCR from Desulfosarcina cetonica slowly disproportionates two 1,5-dienoyl-CoA to BzCoA and 1-monenoyl-CoA at about 0.1% of the 1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity rate. Assays for BzCoA reduction are perfomred by flavin-based electron bifurcation
-
-
-
additional information
?
-
-
benzoyl-CoA is dearomatized into cyclohexa-1,5-diene-carboxyl-CoA in an ATP-independent reaction cycle
-
-
?
additional information
?
-
-
in absence of benzoyl-CoA the enzyme exhibits oxygen-sensitive ATPase activity
-
-
?
additional information
?
-
-
no substrate: furan-3-carbonyl-CoA, pyrrole-2-carbonyl-CoA, pyridine-4-carbonyl-CoA, 4-fluoro-benzoyl-CoA
-
-
?
additional information
?
-
-
reduction of aromatic ring requires 2 mol of ATP per mol of benzoyl-CoA
-
-
?
additional information
?
-
enzyme MBRTcl preferentially dearomatizes meta-substituted BzCoA analogues containing methyl-, chloro-, or hydroxy-functionalities. With these substrates, relative specific activities compared with BzCoA are substantially higher with enzyme MBRTcl than with BCRTar from Thauera aromatica. MBRTcl also converts para-substituted halo- and methyl-BzCoA analogues that are not converted by BCRTar. Exceptions are 3-fluoro- and 4-fluorobenzoyl-CoA that serve as substrates for both enzymes. Neither of the two enzymes reduces heterocyclic nicotinoyl-CoA, and 4-hydroxybenzoyl-CoA is a poor substrate for both enzymes. Enzyme BCRTar also shows no or poor activity with 4-methylbenzoyl-CoA, 4-ethylbenzoyl-CoA, 4-chlorobenzoyl-CoA, 2-bromobenzoyl-CoA, 4-bromobenzoyl-CoA, and 2-hydroxybenzoyl-CoA. Formation of the corresponding two electron-reduced 1,5-dienoyl-CoA analogues is confirmed by ESI-Q-TOF-MS analysis, substrate specificity, overview. The activity enzyme assay uses Ti(III) citrate as artificial electron donor
-
-
-
additional information
?
-
-
enzyme MBRTcl preferentially dearomatizes meta-substituted BzCoA analogues containing methyl-, chloro-, or hydroxy-functionalities. With these substrates, relative specific activities compared with BzCoA are substantially higher with enzyme MBRTcl than with BCRTar from Thauera aromatica. MBRTcl also converts para-substituted halo- and methyl-BzCoA analogues that are not converted by BCRTar. Exceptions are 3-fluoro- and 4-fluorobenzoyl-CoA that serve as substrates for both enzymes. Neither of the two enzymes reduces heterocyclic nicotinoyl-CoA, and 4-hydroxybenzoyl-CoA is a poor substrate for both enzymes. Enzyme BCRTar also shows no or poor activity with 4-methylbenzoyl-CoA, 4-ethylbenzoyl-CoA, 4-chlorobenzoyl-CoA, 2-bromobenzoyl-CoA, 4-bromobenzoyl-CoA, and 2-hydroxybenzoyl-CoA. Formation of the corresponding two electron-reduced 1,5-dienoyl-CoA analogues is confirmed by ESI-Q-TOF-MS analysis, substrate specificity, overview. The activity enzyme assay uses Ti(III) citrate as artificial electron donor
-
-
-
additional information
?
-
-
the enzyme catalyzes ATP-dependent reductive dehalogenation of 3-chloro/3-bromobenzoyl-CoA to benzoyl-CoA, whereas 3-fluorobenzoyl-CoA is converted to a fluorinated cyclic dienoyl-CoA compound. Reductive dechlorination/debromination of 3-Cl-/3-Br-benzoyl-CoA catalyzed by class I BCR yielding benzoyl-CoA, the latter is subsequently dearomatized by the same enzyme. The elimination of HCl/HBr may occur enzymatically or spontaneously
-
-
?
additional information
?
-
-
reactions under anaerobic conditions
-
-
?
additional information
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enzyme MBRTcl preferentially dearomatizes meta-substituted BzCoA analogues containing methyl-, chloro-, or hydroxy-functionalities. With these substrates, relative specific activities compared with BzCoA are substantially higher with enzyme MBRTcl than with BCRTar from Thauera aromatica. MBRTcl also converts para-substituted halo- and methyl-BzCoA analogues that are not converted by BCRTar. Exceptions are 3-fluoro- and 4-fluorobenzoyl-CoA that serve as substrates for both enzymes. MBRTcl appears to be less sensitive to steric effects of meta- and para-positioned substituents than BCRTar. But neither of the two enzymes reduces heterocyclic nicotinoyl-CoA, and 4-hydroxybenzoyl-CoA is a poor substrate for both enzymes. Formation of the corresponding two electron-reduced 1,5-dienoyl-CoA analogues is confirmed by ESI-Q-TOF-MS analysis, substrate specificity, overview. The activity enzyme assay uses Ti(III) citrate as artificial electron donor
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additional information
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enzyme MBRTcl preferentially dearomatizes meta-substituted BzCoA analogues containing methyl-, chloro-, or hydroxy-functionalities. With these substrates, relative specific activities compared with BzCoA are substantially higher with enzyme MBRTcl than with BCRTar from Thauera aromatica. MBRTcl also converts para-substituted halo- and methyl-BzCoA analogues that are not converted by BCRTar. Exceptions are 3-fluoro- and 4-fluorobenzoyl-CoA that serve as substrates for both enzymes. MBRTcl appears to be less sensitive to steric effects of meta- and para-positioned substituents than BCRTar. But neither of the two enzymes reduces heterocyclic nicotinoyl-CoA, and 4-hydroxybenzoyl-CoA is a poor substrate for both enzymes. Formation of the corresponding two electron-reduced 1,5-dienoyl-CoA analogues is confirmed by ESI-Q-TOF-MS analysis, substrate specificity, overview. The activity enzyme assay uses Ti(III) citrate as artificial electron donor
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the enzyme catalyzes ATP-dependent reductive dehalogenation of 3-chloro/3-bromobenzoyl-CoA to benzoyl-CoA, whereas 3-fluorobenzoyl-CoA is converted to a fluorinated cyclic dienoyl-CoA compound. Reductive dechlorination/debromination of 3-Cl-/3-Br-benzoyl-CoA catalyzed by class I BCR yielding benzoyl-CoA, the latter is subsequently dearomatized by the same enzyme. The elimination of HCl/HBr may occur enzymatically or spontaneously
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reactions under anaerobic conditions
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