1.3.8.1: short-chain acyl-CoA dehydrogenase

This is an abbreviated version!
For detailed information about short-chain acyl-CoA dehydrogenase, go to the full flat file.

Word Map on EC 1.3.8.1

1.3.8.1

ethylmalonic

medium-chain

dehydrogenases

beta-oxidation

inborn

aciduria

carnitine

acylcarnitines

scadd

butyrylcarnitine

methylsuccinic

isobutyryl-coa

isovaleryl-coa

phenylketonuria

vlcad

very-long-chain

isovaleric

crotonyl-coa

octanoyl-coas

megasphaera

3-methylcrotonyl-coa

elsdenii

synthesis

medicine

Reaction

Synonyms

3-hydroxyacyl CoA reductase, ACADS, Acfer_0556, Acfer_1477, acyl-CoA dehydrogenase short chain, BCAD, BCD, bcd2, BcdAf, bSCAD, butyryl CoA dehydrogenase, butyryl coenzyme A dehydrogenase, butyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase complex, butyryl-CoA dehydrogenase/Etf complex, CD1054, dehydrogenase, butyryl coenzyme A, EC 1.3.2.1, EC 1.3.99.2, enoyl-coenzyme A reductase, ethylene reductase, HADHSC, non-dissociating bifurcating Etf-Bcd complex, SCAD, ScdA, short chain acyl-CoA dehydrogenase, short-chain 3-hydroxyacyl-CoA dehydrogenase, short-chain acyl CoA dehydrogenase, short-chain acyl-CoA dehydrogenase, short-chain acyl-coenzyme A dehydrogenase, short-chain acylCoA dehydrogenase, unsaturated acyl coenzyme A reductase

ECTree

1 Oxidoreductases

1.3 Acting on the CH-CH group of donors

1.3.8 With a flavin as acceptor

1.3.8.1 short-chain acyl-CoA dehydrogenase

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Results	in table
3	Activating Compound
7829	AA Sequence
11	Application
1	CAS Registry Number
25	Cloned(Commentary)
30	Cofactor
3	Crystallization (Commentary)
692	Disease/ Diagnostics
2	Expression
22	General Information
2	General Stability
37	Inhibitors
6	kcat/KM [mM/s]
4	Ki Value [mM]
78	KM Value [mM]
23	Localization
3	Metals/Ions
33	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
145	Organism
2	Oxidation Stability
19	Pathway
39	PDB ID
17	pH Optimum
9	pH Range
5	pH Stability
2	pI Value
2	Posttranslational Modification
47	Protein Variants
27	Purification (Commentary)
6	Reaction
3	Reaction Type
102	Reference
1	Renatured (Commentary)
48	Source Tissue
39	Specific Activity [micromol/min/mg]
10	Storage Stability
178	Substrates and Products (Substrate)
19	Subunits
83	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Temperature Stability [°C]
42	Turnover Number [1/s]

Purification

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Purification on EC 1.3.8.1 - short-chain acyl-CoA dehydrogenase

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5 entries

by chromatography and fractionation, to ca. 95% homogeneity

Homo sapiens

676021

by gel filtration, to more than 95% purity

Homo sapiens

672003, 672004

by successive fractionations of anion-exchange chromatography, ammonium sulfate precipitation and gel filtration, to more than 99% homogeneity

Pseudomonas putida

673718

DEAE cellulose, gel filtration, partially purified

Clostridium acetobutylicum

391308

enzyme exhibits a bright green color from a strong absorption band at 710 nm due to tightly bound CoA persulfide

Bos taurus

391294

native enzyme from strain VR4 cell supernatant by ultracentrifugation, ammonium sulfate fractionation, dialysis, anion exchange chromatography, and ultrafiltration, the the enzyme is mixed with excess of solid dithionite under anaerobic conditions to give the yellow form, and further purified by desalting gel filtration under anaerobic conditions

Acidaminococcus fermentans

D2RIQ3, D2RIQ3; D2RL84

742866

preparation of apoprotein

Megasphaera elsdenii

391260

preparation of CoA-persulfide free enzyme

Megasphaera elsdenii

391280

pure enzyme has a characteristic green colour, CoA-persulfide may be the donor in a charge-transfer complex that is responsible for the green colour

Megasphaera elsdenii

391295

Q-Sepharose column chromatography, hydroxyapatite column chromatography, Phenyl-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography

recombinant bSCAD

recombinant SCAD

recombinant wild-type and E367Q mutant enzyme

Megasphaera elsdenii

391302

wild-type enzyme purified, E368Q, E368G and E368L mutant enzyme, partially purified

Rattus norvegicus

391307