1.3.8.7: medium-chain acyl-CoA dehydrogenase

This is an abbreviated version!
For detailed information about medium-chain acyl-CoA dehydrogenase, go to the full flat file.

Reaction

Synonyms

ACAD-9, ACADM, ACD, acyl CoA dehydrogenase, acyl coenzyme A dehydrogenase, acyl dehydrogenase, acyl-CoA dehydrogenase, acyl-CoA dehydrogenase-9, dehydrogenase, acyl coenzyme A, EC 1.3.2.2, EC 1.3.99.3, FadE, fatty acyl coenzyme A dehydrogenase, fatty-acyl-CoA dehydrogenase, general ACAD, general acyl CoA dehydrogenase, LCAD, LipB, MCAD, MCADH, medium chain acyl-CoA dehydrogenase, medium chain-specific acyl-CoA oxidase, medium-chain acyl CoA dehydrogenase, medium-chain acyl-CoA dehydrogenase, medium-chain acyl-coenzyme A dehydrogenase, medium-chain coenzyme A dehydrogenase, pMCAD, PP_1893, PP_2039, PP_2048, PP_2437

ECTree

     1 Oxidoreductases

         1.3 Acting on the CH-CH group of donors

             1.3.8 With a flavin as acceptor

                1.3.8.7 medium-chain acyl-CoA dehydrogenase

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Results	in table
2	Activating Compound
12485	AA Sequence
13	Application
1	CAS Registry Number
19	Cloned(Commentary)
28	Cofactor
8	Crystallization (Commentary)
2577	Disease/ Diagnostics
9	General Information
3	General Stability
58	Inhibitors
14	Ki Value [mM]
117	KM Value [mM]
17	Localization
25	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
120	Organism
10	Pathway
4	PDB ID
7	pH Optimum
4	pH Range
1	pH Stability
78	Protein Variants
29	Purification (Commentary)
9	Reaction
5	Reaction Type
114	Reference
73	Source Tissue
19	Specific Activity [micromol/min/mg]
11	Storage Stability
210	Substrates and Products (Substrate)
19	Subunits
83	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
16	Temperature Stability [°C]
84	Turnover Number [1/s]

Inhibitors

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of the Enzyme Summary Page.

Inhibitors on EC 1.3.8.7 - medium-chain acyl-CoA dehydrogenase

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

(methylenecyclopropyl)acetyl-CoA

2 entries

(R)-(-)-(methylenecyclopropyl)acetyl-CoA

3 entries

(S)-(+)-(Methylenecyclopropyl)acetyl-CoA

Sus scrofa

-

stereospecific inactivation, faster inhibition with (R)-isomer than with (S)-isomer

391311

1-ethyl-3(3-dimethylaminopropyl)-carbodiimide

Sus scrofa

-

90-96% inhibition of activity with electron transfer flavoprotein, not with phenazine methosulfate

391355

2,3-octadienoyl-CoA

Sus scrofa

-

-

391354

2-decenoyl-CoA

Bos taurus

-

medium- and long-chain acyl-CoA dehydrogenase

391283

2-hexadecenoyl-CoA

Rattus norvegicus

-

competitive, long-chain acyl-CoA dehdrogenase

391272

2-octenoyl-CoA

Sus scrofa

-

-

391354

2-octynoyl-CoA

4 entries

3,4-pentadienoyl-CoA

Sus scrofa

-

suicide substrate, 0.1 mM almost complete inhibition

391336

3-butynoyl-CoA

Sus scrofa

-

concentration-dependent, progressive total inactivation, active site inhibitor

391354

3-ketodecanoyl-CoA

Bos taurus

-

medium- and long-chain acyl-CoA dehydrogenase

391283

3-ketooctanoyl-CoA

Sus scrofa

-

76% inhibition

391327

3-oxaoctanoyl-CoA

Sus scrofa

-

-

391321

3-thiaoctanoyl-CoA

Sus scrofa

-

-

391321

4-oxaoctanoyl-CoA

Sus scrofa

-

-

391321

4-thiaoctanoyl-CoA

Sus scrofa

-

-

391321

acetoacetyl-CoA

Sus scrofa

-

-

391358

AgNO3

Rattus norvegicus

-

-

391270

crotonyl-CoA

Sus scrofa

-

-

391320, 391358

CuCl2

Rattus norvegicus

-

-

391270

diethyldicarbonate

Sus scrofa

-

rapid inactivation

391351

DL-3-hydroxydecenoyl-CoA

Bos taurus

-

-

391283

HgCl2

2 entries

iodoacetamide

Homo sapiens

-

2 mM, 73% inhibition

391201

iodoacetic acid

2 entries

L-3-hydroxyoctanoyl-CoA

Sus scrofa

-

0.03 mM, 7% inhibition

391327

Methylmercury chloride

Rattus norvegicus

-

0.1 mM, strong inhibition

391270

Methylmercury iodide

2 entries

N-ethylmaleimide

2 entries

oct-2-yn-4-enoyl-CoA

Rattus norvegicus

-

pH-dependent inactivation

700546

oct-4-en-2-ynoyl-CoA

Rattus norvegicus

-

irreversible inhibitor, active-site directed, enzyme protected by 2-octenoyl-CoA

672585

octanoyl-CoA

Sus scrofa

-

0.0045 mM, 98% inhibition of enoyl-CoA hydratase activity

391329

octenoyl-CoA

Rattus norvegicus

-

competitive, medium-chain acyl-CoA dehydrogenase

391272

octyl-CoA

Sus scrofa

-

-

391354

p-chloromercuribenzoate

3 entries

p-hydroxymercuribenzoate

Rattus norvegicus

-

0.1 mM, almost complete inhibition of the apo-enzyme

391270

S-heptadecyl-CoA

Sus scrofa

-

competitive inhibition

391363

semiquinone electron transfer flavoprotein

Sus scrofa

-

-

391349

-

trans-2-octenoyl-CoA

Sus scrofa

-

0.03 mM, 62% inhibition

391327

trans-3-octenoyl-CoA

Sus scrofa

-

0.03 mM, 67% inhibition

391327

additional information

3 entries

-

(methylenecyclopropyl)acetyl-CoA

Homo sapiens

-

0.01 mM, 95% inhibition

391201

(methylenecyclopropyl)acetyl-CoA

Rattus norvegicus

-

biphasic inhibition of medium-chain acyl-CoA dehydrogenase, rapid initial inactivation for 2 min, half-life: 1.3 min, followed by a slower decline, 95% activity lost after 30 min, no inhibition of long-chain acyl-CoA dehydrogenase

391256

(R)-(-)-(methylenecyclopropyl)acetyl-CoA

Homo sapiens

-

-

391201

(R)-(-)-(methylenecyclopropyl)acetyl-CoA

Sus scrofa

-

0.0155 mM, almost complete inactivation after 5 min, stereospecific inactivation, faster inhibition with (R)-isomer than with (S)-isomer

391311

(R)-(-)-(methylenecyclopropyl)acetyl-CoA

Sus scrofa

-

both (R)- and (S)-stereoisomers are potent inhibitors, inactivation is nonstereospecific

391314

2-octynoyl-CoA

Homo sapiens

-

-

391367

2-octynoyl-CoA

Sus scrofa

-

inactivation of the reduced dehydrogenase may involve a covalent adduct between dihydroflavin and 2-octynoyl-CoA

391317

2-octynoyl-CoA

Sus scrofa

-

mechanism-based inhibitor, suicide-substrate that modifies Glu401

391319

2-octynoyl-CoA

Sus scrofa

-

-

391354, 391367

HgCl2

Rattus norvegicus

-

0.1 mM

391270

HgCl2

Rattus norvegicus

-

-

391271

iodoacetic acid

Sus scrofa

-

S-carboxymethylated enzyme binds octanoyl-CoA but can no longer be reduced by it

391351

iodoacetic acid

Sus scrofa

-

30 mM, half-live: 100 min, loss of 98% activity after 25 h, 0.12 mM octanoyl-CoA strongly protects, S-carboxmethylation of a single catalytically essential methionine inactivates the enzyme

391364

Methylmercury iodide

Rattus norvegicus

-

0.1 mM, strong inhibition, half-time of inactivation: 2 min, octanoyl-CoA protects

391270

Methylmercury iodide

Rattus norvegicus

-

-

391271

N-ethylmaleimide

Homo sapiens

-

2 mM, 43% inhibition

391201

N-ethylmaleimide

Rattus norvegicus

-

inhibitory to apo-enzyme, 2 mM, half-time of inactivation: 4 min, complete inactivation after 20 min

391270

p-chloromercuribenzoate

Homo sapiens

-

0.1 mM, 17% inhibition

391201

p-chloromercuribenzoate

Rattus norvegicus

-

inhibition of apo-enzyme

391270

p-chloromercuribenzoate

Rattus norvegicus

-

-

391271

additional information

Bos taurus

-

not inhibited by ATP, ADP, GTP, GDP, FAD, NAD+, NADP+, carnitine, acetyl-CoA, succinyl-CoA, citrate, isocitrate, succinate, alpha-ketoglutarate, malate, fumarate

391283

-

additional information

Rattus norvegicus

-

not inhibited by saturated and 2,3-unsaturated branched chain acyl-CoA compounds

391272

-

additional information

Rattus norvegicus

-

not inhibited by trans-2,trans-4-octadienoyl-CoA and oct-2-en-4-ynoyl-CoA

672585

-