1.4.1.16: diaminopimelate dehydrogenase
This is an abbreviated version!
For detailed information about diaminopimelate dehydrogenase, go to the full flat file.
Word Map on EC 1.4.1.16
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1.4.1.16
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glutamicum
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corynebacterium
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d-amino
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deamination
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thermophilum
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symbiobacterium
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l-lysine
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dihydrodipicolinate
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2-keto
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thermosphaericus
-
ureibacillus
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isoxazoline
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homoserine
-
sphaericus
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synthesis
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biotechnology
- 1.4.1.16
- glutamicum
- corynebacterium
-
d-amino
-
deamination
- thermophilum
- symbiobacterium
- l-lysine
- dihydrodipicolinate
-
2-keto
- thermosphaericus
-
ureibacillus
-
isoxazoline
- homoserine
- sphaericus
- synthesis
- biotechnology
Reaction
Synonyms
BAB07799, BF3690, DAPDH, DDH, meso-2,6-diaminopimelate dehydrogenase, meso-alpha,epsilon-diaminopimelate dehydrogenase, meso-DAPDH, meso-diaminopimelate dehydrogenase, NADP+-dependent meso-diaminopimelate dehydrogenase, Sth1425, Theth_1310
ECTree
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Purification
Purification on EC 1.4.1.16 - diaminopimelate dehydrogenase
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Ni+-charged chelating Sepharose column chromatography and Toyopearl butyl-650M column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
recombinant enzyme from Escherichia coli strain Rosetta (DE3) by heat treatment and affinity chromatography, native enzyme 47fold to homogeneity by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography, followed by preparative slab PAGE
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recombinant protein
recombinant soluble enzyme 3.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
recombinant wild-type enzyme and mutant Q154L/D158G/T173I/R199M/H249N from Escherichia coli strain Rosetta (DE3) by heat treatment and chelating affinity chromatograhy