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1.4.1.16: diaminopimelate dehydrogenase

This is an abbreviated version!
For detailed information about diaminopimelate dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.16

Reaction

meso-2,6-diaminoheptanedioate
+
H2O
+
NADP+
=
L-2-amino-6-oxoheptanedioate
+
NH3
+
NADPH
+
H+

Synonyms

BAB07799, BF3690, DAPDH, DDH, meso-2,6-diaminopimelate dehydrogenase, meso-alpha,epsilon-diaminopimelate dehydrogenase, meso-DAPDH, meso-diaminopimelate dehydrogenase, NADP+-dependent meso-diaminopimelate dehydrogenase, Sth1425, Theth_1310

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.16 diaminopimelate dehydrogenase

Purification

Purification on EC 1.4.1.16 - diaminopimelate dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, hydroxyapatite, Sephadex G-150
-
Ni+-charged chelating Sepharose column chromatography and Toyopearl butyl-650M column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-NTA column chromatography, and Superdex 200 gel filtration
recombinant enzyme from Escherichia coli strain Rosetta (DE3) by heat treatment and affinity chromatography, native enzyme 47fold to homogeneity by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography, followed by preparative slab PAGE
-
recombinant protein
recombinant soluble enzyme 3.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
recombinant wild-type enzyme and mutant Q154L/D158G/T173I/R199M/H249N from Escherichia coli strain Rosetta (DE3) by heat treatment and chelating affinity chromatograhy