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1.4.1.2: glutamate dehydrogenase

This is an abbreviated version!
For detailed information about glutamate dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.2

Reaction

L-glutamate
+
H2O
 +
NAD+
=
2-oxoglutarate
 +
NH3
 +
NADH
 +
H+

Synonyms

At5g18170, AtGDH1, BpNADGDH, c, CCNA_00086, CsGDH, dehydrogenase, glutamate, GDH, GDH isoenzyme 1, GDH, NAD-dependent, gdh-1, gdh-2, GDH1, GDH2, Gdh2p, GDH3, GdhA, GDHB, GDHI, GdhZ, Glu dehydrogenase, GluD, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase (NAD), glutamate dehydrogenase 2, glutamate dehydrogenase alpha subunit, glutamate dehydrogenase beta subunit, glutamate dehydrogenase isoform 1, glutamate oxidoreductase, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2-nerve-specific GDH, house-keeping GDH, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, More, NAD(+)-dependent glutamate dehydrogenase, NAD(H)-dependent glutamate dehydrogenase, NAD+-dependant glutamate dehydrogenase, NAD+-dependent GDH, NAD+-dependent GDHX, NAD+-dependent GluDH, NAD+-dependent glutamate dehydrogenase, NAD+-GDH, NAD+-glutamate dehydrogenase, NAD+-specific GDH, NAD+-specific glutamate dehydrogenase, NAD-dependent GDH, NAD-dependent glutamate dehydrogenase, NAD-dependent glutamic dehydrogenase, NAD-dependent L-glutamate dehydrogenase, NAD-GDH, NAD-glutamate dehydrogenase, NAD-linked glutamate dehydrogenase, NAD-linked glutamic dehydrogenase, NAD-specific glutamate dehydrogenase, NAD-specific glutamic dehydrogenase, NAD-ylGdh2p, NAD:glutamate oxidoreductase, NADH-dependent GDH, NADH-dependent glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, NADH-linked glutamate dehydrogenase, OsGDH1, OsGDH2, OsGDH3, Pcal_1031, RocG, sco2999, Surface-associated protein PGAG1, t-GDH, type I GDH, YALI0E09603g, ylGDH2

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.2 glutamate dehydrogenase

Cloned

Cloned on EC 1.4.1.2 - glutamate dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and expression of soluble wild-type and mutant enzymes in Escherichia coli strain XL1-Blue
-
expressed in Escherichia coli
expressed in Escherichia coli (DE3)-codon plus-RIL cells
-
expressed in Escherichia coli BL21
-
expressed in Escherichia coli BL21(DE3)/pLysS cells
-
expressed in Escherichia coli strain TG1
-
expressed in Escherichia coli Top10 cells
expressed in Escherichia coli XLOLR
expressed in Solanum lycopersicum line S77-H
-
expression in Escherichia coli
expression in Escherichia coli BL21 cells
expression in Escherichia coli MV1184
expression in Escherichia coli TG1
-
expression in Escherichia coli TG1 cells
-
expression in Gdh-null Saccharomyces cerevisiae
expression of inactive enzyme in Escherichia coli
-
expression of isoenzymes in Escherichia coli as soluble proteins
-
expression of wild-type GDH in Escherichia coli strain BL21(DE3), and of the SeMet-labeled enzyme in Escherichia coli strain B834 (DE3)
gene gdh2, GDH2 is encoded on chromosome IV
gene gdh2, single copy gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression in the monomorphic mutant Y-5 of Benjaminiella poitrasii and in human-pathogenic, non-dimorphic Candida glabrata
gene msmeg_4699, semi-quantitative real-time PCR expression analysis
gene pcal_1031, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of N-terminally Met-Ala-Ser-tagged and C-terminally His6-tagged enzyme in Eschericia coli strain Rosetta(DE3)
-
gene YALI0E09603g, cloned from strain INAG34815, sequence comparisons, recombinant expression in gdh-null Saccharomyces cerevisiae, recombinant overexpression in Yarrowia lipolytica strain under control of the constitutive TEF promoter using the JMP62 expression vectors. GDH activities in Yarrowia lipolytica are differentially expressed, overview
genes gdh1, gdh2 and gdh3, GDH1 gene encodes a polypeptide subunit termed beta and GDH2 gene encodes alpha and the polypeptides can be assembled as homo- or heterohexamers composed of different ratios of alpha and beta, leading to the formation of seven active isoenzymes
-
ligated to NdeI/BamHI digested pET3a, expression in Escherichia coli BL21 (DE3)
ligated to plasmid pT7Blue vector and cloned into Escherichia coli DH5a
overexpressed in Escherichia coli
-
overexpression in Escherichia coli
-
overexpression in halophilic host Haloferax volcanii strain DS70 of soluble, active enzyme with NAD+-GDH activity about 800times higher in the induced sample as compared to controls, expression in inclusion bodies in mesophilic Escherichia coli
recombinant expression in Escherichia coli TOP10 cells
recombinant expression of wild-type and mutant enzymes
-
three genes encoding the alpha-subunit, Slgdh-NAD;A1-3, and one additional gene encoding the beta-subunit of GDH, Slgdh-NAD;B1, Slgdh-NAD;A1-3 show conserved structures, whereas Slgdh-NAD;B1 includes a 5'-untranslated exon, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expresssion analysis
-
tobacco transformed with either an antisense or sense copy of a beta-subunit gene of tomato GDH, transgenic plants recover with between 0.5- and 34-times normal leaf GDH activity