1.4.1.3: glutamate dehydrogenase [NAD(P)+] This is an abbreviated version! For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file .
Reaction
L-glutamate +
H2O +
NAD(P)+ =
2-oxoglutarate +
NH3 +
NAD(P)H +
H+
Synonyms At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH
ECTree
Cofactor
Cofactor on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]
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NAD+
-
-
NAD+
-
no activity with NADP+
NAD+
-
glutamate dehydrogenase 2 uses only NAD+ in the deamination direction
NAD+
-
2 times higher reaction rate than with NADP+
NAD+
-
activity with NAD+ is 3-5fold higher than using NADP+
NAD+
utilizes NAD+ for nitrogen liberation
NAD+
GDH1 uses NAD+ or NADP+ as coenzyme
NAD+
-
both NAD+ and NADP+ are utilized as cofactors. The inactive enzyme also binds cofactors
NADH
-
-
NADH
-
preferred cofactor
NADH
-
reaction rate NADH/NADPH: 6
NADH
-
ratio of NADH/NADPH-dependent activity: 1/1.6
NADH
-
reaction rate NADH/NADPH: 4.5 (GDH2)
NADH
-
reaction rate NADH/NADPH: 1.3 (GDH1)
NADH
-
reaction rate NADH/NADPH: 15
NADP+
-
-
NADP+
-
no activity with NAD+
NADP+
-
only 5% of the activity with NAD+
NADP+
-
with NADP+ initial rates are much slower than with NAD+
NADP+
-
enzyme activity with NADP+ is higher than the activity with NAD+
NADP+
-
lower activity with NADP+ compared to NAD+
NADP+
utilizes NADP+ for nitrogen incorporation
NADP+
GDH1 uses NAD+ or NADP+ as coenzyme
NADP+
-
both NAD+ and NADP+ are utilized as cofactors. The inactive enzyme also binds cofactors
NADPH
-
-
NADPH
-
reaction rate NADH/NADPH: 6
NADPH
-
reaction rate NADH/NADPH: 15
NADPH
-
glutamate dehydrogenase 2, reaction rate NADH/NADPH: 4.4
NADPH
-
3fold higher activity than with NADH
NADPH
-
glutamate dehydrogenase 1, reaction rate NADH/NADPH: 1.3
NADPH
-
NADPH/NADH ratio of reaction rate: 10/1, NAD+/NADH ratio of reaction rate: 5/1
NADPH
-
amination activity is 5fold higher with NADPH than that with NADH
NADPH
-
at lower maximal rate than with NADH
additional information
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2 soybean isoenzymes with different coenzyme specificities, glutamate dehydrogenase 1: active with NADH, NAD+, NADPH and NADP+, glutamate dehydrogenase 2: active with NADH, NAD+, NADPH but not NADP+
-
additional information
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cofactor not mentioned
-
additional information
cofactor specificity not mentioned
-
additional information
cofactor specificity not mentioned
-
additional information
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cofactor specificity not mentioned
-
additional information
cofactor specificity not mentioned
-
additional information
cofactor specificity not mentioned
-
additional information
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cofactor specificity not mentioned
-
additional information
cofactor specificity not mentioned
-
additional information
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GDH is a dual-cofactor GDH
-
additional information
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GDH is a dual-cofactor GDH, with activity that is greater with NAD+/NADH than with NADP+/NADPH
-
additional information
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the enzyme can use both NAD+ and NADP+ as electron acceptors, displaying more affinity for NADP+ than for NAD+. No activity with NADH or NADPH
-
additional information
-
the similarity in relative activation when both cofactors are present, combined with consistently greater GDH product formation from equimolar NADH than with NADPH, does not support the idea that there is a preferential utilization of NADPH by bovine GDH. In the reductive amination direction, the rates of product formation are always greater for NADH oxidation than NADPH oxidation
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additional information
coenzyme specificity of GDH for NADPH and NADH
-