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1.4.1.3: glutamate dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

L-glutamate
+
H2O
+
NAD(P)+
=
2-oxoglutarate
+
NH3
+
NAD(P)H
+
H+

Synonyms

At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.3 glutamate dehydrogenase [NAD(P)+]

Storage Stability

Storage Stability on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°, stable for several months
dogfish
-
-18°C, several months, no loss of activity
-
-20°C, 4°C, 25°C, 6 months, 5% loss of activity
-
-70°C, stable for 6 months
-
0°C, 200 mM phosphate buffer, pH 7.4, several weeks, very little loss of activity
-
0°C, 50 mM Na,K phosphate buffer, pH 5.5, stable
-
25°C, soluble enzyme in the presence of 25% v/v acetone at pH 7, 1 week, no loss of activity
-
4°C, 1 mg/ml protein concentration, glutamate dehydrogenase remains stable for at least one month
-
4°C, 20% loss of activity after 40 days
-
4°C, 6 months, stable
-
4°C, more than 20 days, no loss of activity
-
5°, 28% Na2SO4 solution, several weeks, very little loss of activity
-
5°C, sodium phosphate buffer pH 6.8, several months, no loss of activity
-
suspended in ammonium sulfate, stable for months
-