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1.4.1.8: valine dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about valine dehydrogenase (NADP+), go to the full flat file.

Reaction

L-valine
+
H2O
+
NADP+
=
3-methyl-2-oxobutanoate
+
NH3
+
NADPH
+
H+

Synonyms

dehydrogenase, valine (nicotinamide adenine dinucleotide phosphate), ValDH, valine dehydrogenase (nicotinamide adenine dinucleotide phosphate), VDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.8 valine dehydrogenase (NADP+)

Purification

Purification on EC 1.4.1.8 - valine dehydrogenase (NADP+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
143fold purification
-
1508fold purification
-
180fold purification
-
21fold purification
-
His-bind resin column, 95% purity
-
shoots: 21fold purification, roots: 3.9fold purification
-
soluble enzyme from roots, 3.9fold purification
-
VDH and A124G mutant, His-bind resin column  
-
VDH2, 346fold purification
-
VDH2, 52.6fold purification
-