1.4.1.8: valine dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about valine dehydrogenase (NADP+), go to the full flat file.

dehydrogenase, valine (nicotinamide adenine dinucleotide phosphate), ValDH, valine dehydrogenase (nicotinamide adenine dinucleotide phosphate), VDH

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Results	in table
7	Activating Compound
15	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
83	Cofactor
1	General Stability
33	Inhibitors
1	Ki Value [mM]
90	KM Value [mM]
22	Localization
14	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
51	Organism
26	pH Optimum
2	pH Range
3	pH Stability
7	Protein Variants
12	Purification (Commentary)
2	Reaction
5	Reaction Type
35	Reference
25	Source Tissue
41	Specific Activity [micromol/min/mg]
8	Storage Stability
282	Substrates and Products (Substrate)
11	Subunits
5	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
1	Temperature Stability [°C]
20	Turnover Number [1/s]

Storage Stability on EC 1.4.1.8 - valine dehydrogenase (NADP+)

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-20°C, 0.2 M Tris-HCl buffer, pH 7.4, 3 months, no loss of activity

-20°C, 50 mM Tris-HCl buffer, pH 7.4, 5 mM 2-mercaptoethanol, 1 mM EDTA, 30% v/v glycerol, 24 h, 90% loss of activity

-20°C, 70% (NH4)2SO4 suspension in 0.2 M Tris-HCl buffer, 5 months, stable

-70°C, 25 mM sodium phosphate buffer pH 7, equal volume of glycerol, 3 months, stable

-80°C, 100 mM Tris-HCl buffer, pH 7.4, 20% v/v glycerol, 2 months, stable

-80°C, 50 mM potassium phosphate pH 7.5, 10% v/v glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, 1 month, loss of specific activity

4°C, 0.2 M Tris-HCl buffer, pH 7.4, 24 h, no loss of activity

4°C, 50 mM Tris-HCl buffer, pH 7.4, 5 mM 2-mercaptoethanol, 1 mM EDTA, 10% v/v glycerol, 48 h, stable