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1.4.7.1: glutamate synthase (ferredoxin)

This is an abbreviated version!
For detailed information about glutamate synthase (ferredoxin), go to the full flat file.

Word Map on EC 1.4.7.1

Reaction

2 L-glutamate +

oxidized ferredoxin
=
L-glutamine
+
2-oxoglutarate
+
reduced ferredoxin
+ 2 H+

Synonyms

abc1, At5g04140, ES7, Fd-dependent glutamate synthase, Fd-GOGAT, Fd-GOGAT1, FdGOGAT, ferredoxin-dependent GltS, ferredoxin-dependent glutamate synthase, ferredoxin-dependent glutamate synthase1, ferredoxin-dependent glutamine 2-oxoglutarate aminotransferase, ferredoxin-dependent GOGAT, ferredoxin-glutamate synthase, ferredoxin-GOGAT, gltB, GltS, Glu1, glutamate synthase, glutamate synthase (ferredoxin-dependent), glutamine 2-oxoglutarate amidotransferase, glutamine:2-oxoglutarate amidotransferase, glutamine:oxoglutarate amidotransferase, GOGAT, lc7

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.7 With an iron-sulfur protein as acceptor
                1.4.7.1 glutamate synthase (ferredoxin)

Purification

Purification on EC 1.4.7.1 - glutamate synthase (ferredoxin)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on ferredoxin Sepharose
ammonium sulfate precipitation, hydroxyapatite column chromatography, butyl-Toyopearl column chromatography, MonoQ column chromatography, and DEAE-Toyopearl column chromatography
ammonium sulfate, DEAE cellulose, glutamate agarose
-
ammonium sulfate, DEAE-cellulose, DEAE-Sephacel, Phenyl-Sepharose
-
ferredoxin-immobilized Sepharose column chromatography
-
Ni-NTA column chromatography, Sepharose column chromatography, and Superdex 200 gel filtration
partially purified
-
protamine sulfate, DEAE-Sephacel, hydroxylapatite, ferredoxin-Sepharose
-
recombinant enzyme
-
recombinant enzyme expressed in Escherichia coli
recombinant enzyme, affinity chromatography on ferredoxin-Sepharose, native enzyme, partially purified
-