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1.4.9.1: methylamine dehydrogenase (amicyanin)

This is an abbreviated version!
For detailed information about methylamine dehydrogenase (amicyanin), go to the full flat file.

Word Map on EC 1.4.9.1

Reaction

methylamine
+
H2O
 + 2 amicyanin =
formaldehyde
 +
NH3
 + 2 reduced amicyanin

Synonyms

amine dehydrogenase, amine: oxidoreductase (acceptor deaminating), dehydrogenase, amine, EC 1.4.98.1, EC 1.4.99.3, Heme 2, MADH, mauA, methylamine dehydrogenase, primary-amine dehydrogenase, QH-AmDH, QHNDH, quinohaemoprotein amine dehydrogenase, quinohemoprotein amine dehydrogenase, quinohemoprotein amine dehydrogenases, sQH-AmDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.9 With a copper protein as acceptor
                1.4.9.1 methylamine dehydrogenase (amicyanin)

Cloned

Cloned on EC 1.4.9.1 - methylamine dehydrogenase (amicyanin)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
-
expressed in Rhodobacter sphaeroides
Expression in Escherichia coli, the production of knock out-mutants unable to catabolize 2-phenylethylamine is carried out by mutagenesis.
-
genes qhpA, qhpB, and qhpC encoding the alpha, beta, and gamma subunits of QHNDH, respectively. The structural genes encoding the three QHNDH subunits constitute an operon harboring six apparent open reading frames (ORFs) that are transcribed in a coordinated manner upon addition of amines to the culture medium. qhpADCBEF genes constitute a hexacistronic operon. DNA and amino acid sequence determination and analysis, and reverse transcription PCR expression analysis. Regulation of qhp genes, overview
heterologous expression of cytochrome c-550 in Escherichia coli
-
heterologous expression of mutated alpha subunit in Rhodobacter sphaeroides
-
MADH is a heterotetramer consisting of two alpha subunits and two beta subunits which are encoded by mauB and mauA, respectively
PreMADH can be generated by expression of recombinant MADH in the background of a mauG deletion
-
recombinant expression of pre-MADH in Rhodobacter sphaeroides
the methylamine utilization (mau) gene cluster contains 11genes, two of which encode the 42.5 kDa alpha-(mauB) and the 14.2 kDa beta-subunits (mauA) of MADH, recombinant co-overexpression of genes mauAB in Rhodobacter sphaeroides in the presence of mauDEFG, the four genes required for MADH maturation results in an alpha2beta2 MADH protein that has no catalytic activity, because the protein contains all six beta-subunit disulfides, but has a partially synthesized TTQ cofactor with only a single -OH group added to the indole of betaTrp57 (betaTrp57-OH)