1.4.9.1: methylamine dehydrogenase (amicyanin)

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For detailed information about methylamine dehydrogenase (amicyanin), go to the full flat file.

Reaction

Synonyms

amine dehydrogenase, amine: oxidoreductase (acceptor deaminating), dehydrogenase, amine, EC 1.4.98.1, EC 1.4.99.3, Heme 2, MADH, mauA, methylamine dehydrogenase, primary-amine dehydrogenase, QH-AmDH, QHNDH, quinohaemoprotein amine dehydrogenase, quinohemoprotein amine dehydrogenase, quinohemoprotein amine dehydrogenases, sQH-AmDH

ECTree

     1 Oxidoreductases

         1.4 Acting on the CH-NH₂ group of donors

             1.4.9 With a copper protein as acceptor

                1.4.9.1 methylamine dehydrogenase (amicyanin)

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Results	in table
14	Activating Compound
573	AA Sequence
1	Application
2	CAS Registry Number
12	Cloned(Commentary)
38	Cofactor
16	Crystallization (Commentary)
1	Expression
9	General Information
4	General Stability
35	Inhibitors
88	KM Value [mM]
4	Localization
14	Metals/Ions
38	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
104	Organism
4	Pathway
20	PDB ID
9	pH Optimum
1	pH Range
2	pH Stability
7	Posttranslational Modification
20	Protein Variants
20	Purification (Commentary)
5	Reaction
5	Reaction Type
67	Reference
1	Source Tissue
8	Specific Activity [micromol/min/mg]
1	Storage Stability
104	Substrates and Products (Substrate)
26	Subunits
64	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
4	Temperature Stability [°C]
64	Turnover Number [1/s]

Natural Substrates Products

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of the Enzyme Summary Page.

Natural Substrates Products on EC 1.4.9.1 - methylamine dehydrogenase (amicyanin)

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

2-phenylethylamine + 2 H2O + 2 acceptor

2-phenylacetic acid + NH3 + 2 reduced acceptor

Pseudomonas putida

-

primary amine

686425

-

-

?

methylamine + amicyanin + H2O

formaldehyde + reduced amicyanin + NH3

Paracoccus versutus

-

amicyanin is the in vivo electron acceptor

396582

-

-

?

methylamine + H2O + 2 amicyanin

formaldehyde + NH3 + 2 reduced amicyanin

3 entries

methylamine + H2O + amicyanin

formaldehyde + NH3 + reduced amicyanin

Paracoccus denitrificans

Q51658

-

723880

-

-

?

additional information

?

-

3 entries

methylamine + H2O + 2 amicyanin

formaldehyde + NH3 + 2 reduced amicyanin

Paracoccus denitrificans

P22619; P29894

-

741759, 742302, 742338

-

-

?

methylamine + H2O + 2 amicyanin

formaldehyde + NH3 + 2 reduced amicyanin

Paracoccus denitrificans

P22619

-

763938, 765329

-

-

?

methylamine + H2O + 2 amicyanin

formaldehyde + NH3 + 2 reduced amicyanin

Paracoccus denitrificans

-

-

765329

-

-

?

additional information

?

-

Paracoccus denitrificans

-

amicyanin ami catalyzes the electron transfer from MADH to the terminal oxidase, without the need for any c-type cytochrome. In the absence of either MADH or cytochrome aa3, amicanin is not capable of oxygen reduction on the same time scale. The oxygen consumption depends nearly linearly on the amicyanin concentration up to at least 100 microM. Experiments demonstrate a remarkable number of possibilities for the electron transfer. The interactions appear to be governed exclusively by the electrostatic nature of each of the proteins. Paracoccus denitrificans provides a pool of cytochromes for efficient electron transfer via weak, ill-defined interactions

715290

-

-

?

additional information

?

-

Pseudomonas putida

-

an essential enzyme for the aerobic degradation of many primary amines even though they have quite different chemical structures (aromatic or aliphatic)

686425

-

-

?

additional information

?

-

Pseudomonas putida

-

when different QHNDH mutants (peaA, peaC and peaD) are transformed with a genetic construction containing the peaABCD cluster, all the recombinant strains efficiently catabolized 2-phenylethylamine as well as other primary amines like propyl-, butyl- and pentylamine.

686425

-

-

?