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1.5.1.20: methylenetetrahydrofolate reductase [NAD(P)H]

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate reductase [NAD(P)H], go to the full flat file.

Word Map on EC 1.5.1.20

Reaction

5-methyltetrahydrofolate
+
NAD(P)+
=
5,10-methylenetetrahydrofolate
 +
NAD(P)H
 +
H+

Synonyms

10-methylenetetrahydrofolate reductase, 5,10-CH2-H4folate reductase, 5,10-methylenetetrahydrofolate reductase, 5,10-methylenetetrahydrofolate reductase (FADH2), 5,10-methylenetetrahydrofolate reductase (NADPH), 5,10-methylenetetrahydrofolic acid reductase, 5,10-methylenetetrahydropteroylglutamate reductase, 5-methylenetetrahydrofolate:NADP+ oxidoreductase, 5-methyltetrahydrofolate:(acceptor) oxidoreductase, 5-methyltetrahydrofolate:NAD oxidoreductase, 5-methyltetrahydrofolate:NAD+ oxidoreductase, 5-methyltetrahydrofolate:NADP+ oxidoreductase, AtMTHFR-1, EC 1.1.1.171, EC 1.1.1.68, EC 1.1.99.15, EC 1.7.99.5, HsMTHFR, MET13, methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide) reductase, methylenetetrahydrofolate reductase, methylenetetrahydrofolate reductase (NADPH), methylenetetrahydrofolic acid reductase, MetVF-type methylenetetrahydrofolate reductase, More, MTHFR, MTHFR2, N5,10-methylenetetrahydrofolate reductase, N5,N10-methylenetetrahydrofolate reductase, NADH:CH2-H4folate oxidoreductase, NapAB, reductase, methylenetetrahydrofolate (reduced nicotinamide adenine dinucleotide phosphate), reductase, methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide), respiratory nitrate reductase, type II-MTHFR

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.20 methylenetetrahydrofolate reductase [NAD(P)H]

Purification

Purification on EC 1.5.1.20 - methylenetetrahydrofolate reductase [NAD(P)H]

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
20fold purification
-
62fold purification of recombinant enzyme, expressed in Sf9 cells from Spodoptera frugiperda
-
643fold purification of recombinant enzyme, expressed in Saccharomyces cerevisiae
-
about 100fold purification
-
homogeneity
more than 100fold partial purification
-
native enzyme 57.6fold from cytoplasm by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration
purification of wild-type and histidine-tagged D120N and E28Q mutant enzymes
-
recombinant enzyme from strain BL21(DE3)
-
salting out and DEAE Sepharose column chromatography
-
Toyopearl SuperQ-650M column chromatography and nickel affinity column chromatography