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1.5.1.3: dihydrofolate reductase

This is an abbreviated version!
For detailed information about dihydrofolate reductase, go to the full flat file.

Word Map on EC 1.5.1.3

Reaction

5,6,7,8-tetrahydrofolate
+
NADP+
=
7,8-dihydrofolate
 +
NADPH
 +
H+

Synonyms

5,6,7,8-tetrahydrofolate: NADP+ oxidoreductase, 7,8-dihydrofolate reductase, At2g16370, At4g34570, bifunctional dihydrofolate reductase-thymidylate synthase, bifunctional TS-DHFR, BmDHFR, dehydrogenase, tetrahydrofolate, DFR-TS, DFR1, DfrA, DfrB, DHFR, DHFR type IIIC, DHFR-TS, DHFR-TS1, DHFR-TS2, DHFR2, DHFRL1, DHFRLS, dihydrofolate reductase, dihydrofolate reductase-like, dihydrofolate reductase-thymidylate synthase, dihydrofolate reductase:thymidylate synthase, dihydrofolic acid reductase, dihydrofolic reductase, EC 1.5.1.4, ecDHFR, folA, folA3, folic acid reductase, folic reductase, FolM, hDHFR, hDHFR-1, hDHFR-2, HjDHFR, hvDHFR1, hvDHFR2, LAU_0427, LBRM_06_0830, mDHFR, mjDHFR, More, myDHFR, NADPH-dihydrofolate reductase, pcDHFR, PKNH_0509600, ppDHFR, pteridine reductase, pteridine reductase:dihydrofolate reductase, PTR2, R-plasmid-encoded dihydrofolate reductase, R67 DHFR, R67 dihydrofolate reductase, reductase, dihydrofolate, S3DHFR, Smdhfr, Smp 175230, spDHFR, svDHFR, tcptr1, tetrahydrofolate dehydrogenase, THY-1, THY-2, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase-dihydrofolate reductase, Trimethoprim resistance protein, TS-DHFR, WUBG_00817

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.3 dihydrofolate reductase

Cloned

Cloned on EC 1.5.1.3 - dihydrofolate reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a baculovirus system is established for the expression of this DHFR in Bombyx mori chrysalides. To isolate the expressed protein, whole infected pupae are homogenized
a dihydrofolate reductase-like sequence, DHFRLS, is encoded on chromosome 4, orfs 3139757-3140178 and 413244-415704, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis. RT-qPCR analysis shows no evidence of expression of the DHFRLS in rat
a dihydrofolate reductase-like sequence, DHFRLS, is encoded on chromosome 9, orf 31567433:31568480, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis
a variant (Xet-3) of dihydrofolate reductase from Escherichia coli constructed by introducing residues located at the Thermotoga maritima dihydrofolate reductase (TmDHFR) dimer interface is expressed in Escherichia coli BL21(DE3) cells
-
amplification and DNA-sequencing of full length gene from DNA-library
-
cloning of gene encoding mutant R53M enzyme from methotrexate-resistant strain into an expression plasmid, expression in Escherichia coli
-
DNA-sequence analysis
-
expresion in Escherichia coli and CHO cell
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli HB101 cells
expressed in Escherichia coli JM109 cells
-
expressed in Escherichia coli strain ER2566 bearing a deletion of the DHFR gene (folA) and thymidylate synthethase (thyA) as a His-tagged fusion protein
expressed in inclusion bodies in high yield in Escherichia coli under the control of the T7 promoter
expression as His-tagged enzyme in Escherichia coli strain BL21(DE3)
expression in BL21 cell
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3) pLysS
-
expression in Escherichia coli including 13 amino acid loop
expression in Escherichia coli of wild-type and mutant enzymes
-
expression in Escherichia coli using the multicopy vector pBR322
-
expression in H-1299 cell
-
expression in Saccharomyces cerevisiae
expression of dhfr gene in Escherichia coli
-
expression of His-tagged enzyme in Escherichia coli
expression of His-tagged mutant V77A/I130M/I138V in Escherichia colii strain M15
expression of wild-type and mutant enzymes in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (AI)
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
folA gene, into multicopy vector pBR322, gene enrichment by Mu-mediated transposition
-
gene DHFR, recombinant expression of His-tagged enzyme in Escherichia coli
gene dhfr-ts, DNA and amino acid sequence determination and analysis, a 1,548-bp open reading frame encoding a 58.8-kDa translated peptide containing DHFR and TS domains linked together in a single polypeptide chain, expression of the GST-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein, expression as GFP-tagged enzyme in Babesia gibsoni cytoplasm
gene ptr2, recombinant overexpression of N-terminally His6-tagged enzyme in Escherichia coli
gene Smdhfr, single-copy gene, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
high expression rate of wild-type and mutants from expression vector pCOCK
-
human-derived gene is expressed in Escherichia coli
-
overexpression in Escherichia coli
overexpression in Escherichia coli, regulation by temperature-sensitive repressor of the promotor
-
overexpression of hdrB gene in Escherichia coli
-
overexpression of His-tagged wild-type and mutant enzmyes in Escherichia coli strain BL21(DE3) as soluble proteins
overexpression of wild-type and mutant enzymes in Escherichia coli
-
phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis
quantitative real-time PCR expression analysis of DHFR
-
rat-derived gene is overexpressed in Escherichia coli
-
recombinant bifunctional enzyme DHFR-TS, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain M15(pREP4)
recombinant expression as His-tagged enzyme
recombinant expression of bifunctional Trypanosoma brucei DHFR-TS in form of a fusion protein incorporating Escherichia coli elongation factor Ts (Tsf), in Escherichia coli strain BL21 Star (DE3). The Tsf open reading frame is amplified by PCR from the genomic DNA of Eschrichia coli strain K12, cloning procedure overview. Expression constructs carrying a thymidylate synthase (TS) domain from Trypanosoma brucei, Leishmania major and Homo sapiens are expressed in a TS-deficient Escherichia coli strain (thyA-), while TbDHFR without thymidylate synthase is expressed in the parental thyA+ strain
recombinant expression of C-terminally StrepII-tagged DHFR in Escherichia coli strain BL21(DE3)pLysS
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Rosetta Gami B (DE3)
recombinant expression of His-tagged wild-type enzyme in Escherichia coli Rosetta Gami B (DE3)
recombinant expression of His-tagged wild-type mDHFR and modified mDHFR-43pEthF enzymes in Escherichia coli strain XL1-Blue
recombinant expression of the enzyme
-
review
-
sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
transformation of murine dihydrofolate reductase cDNA by a series of nested PCRs to reproduce the amino acid coding sequence for bovine DHFR, which differs from the murine sequence by 19 amino acids. Expression of the bovine dihydrofolate reductase cDNA in bacterial cells produces a stable recombinant protein with high enzymatic activity and kinetic properties similar to those previously reported for the native protein