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1.5.1.3: dihydrofolate reductase

This is an abbreviated version!
For detailed information about dihydrofolate reductase, go to the full flat file.

Word Map on EC 1.5.1.3

Reaction

5,6,7,8-tetrahydrofolate
+
NADP+
=
7,8-dihydrofolate
 +
NADPH
 +
H+

Synonyms

5,6,7,8-tetrahydrofolate: NADP+ oxidoreductase, 7,8-dihydrofolate reductase, At2g16370, At4g34570, bifunctional dihydrofolate reductase-thymidylate synthase, bifunctional TS-DHFR, BmDHFR, dehydrogenase, tetrahydrofolate, DFR-TS, DFR1, DfrA, DfrB, DHFR, DHFR type IIIC, DHFR-TS, DHFR-TS1, DHFR-TS2, DHFR2, DHFRL1, DHFRLS, dihydrofolate reductase, dihydrofolate reductase-like, dihydrofolate reductase-thymidylate synthase, dihydrofolate reductase:thymidylate synthase, dihydrofolic acid reductase, dihydrofolic reductase, EC 1.5.1.4, ecDHFR, folA, folA3, folic acid reductase, folic reductase, FolM, hDHFR, hDHFR-1, hDHFR-2, HjDHFR, hvDHFR1, hvDHFR2, LAU_0427, LBRM_06_0830, mDHFR, mjDHFR, More, myDHFR, NADPH-dihydrofolate reductase, pcDHFR, PKNH_0509600, ppDHFR, pteridine reductase, pteridine reductase:dihydrofolate reductase, PTR2, R-plasmid-encoded dihydrofolate reductase, R67 DHFR, R67 dihydrofolate reductase, reductase, dihydrofolate, S3DHFR, Smdhfr, Smp 175230, spDHFR, svDHFR, tcptr1, tetrahydrofolate dehydrogenase, THY-1, THY-2, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase-dihydrofolate reductase, Trimethoprim resistance protein, TS-DHFR, WUBG_00817

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.3 dihydrofolate reductase

Storage Stability

Storage Stability on EC 1.5.1.3 - dihydrofolate reductase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 20 mM potassium phosphate, pH 7.0, 0.1 mM EDTA, 10 mM dithiothreitol, 0.2 M KCl, 20% glycerol, mutant S108Q loss of 40% activity after 7 months
-
-20°C, 20 mM potassium phosphate, pH7.0, 0.1 mM EDTA, 10 mM dithiothreitol, 0.2 M KCl, 20% glycerol, mutants S108G, S108A and S108C stable over 7 months
-
-20°C, 40% glycerol, pH 7.4, 5 mM dithiothreitol, 1 mM EDTA, 1 month
-
-20°C, 50% glycerol, 0.1 M potassium phosphate, pH 7.0, less than 10% loss of activity, 6 months
-
-20°C, 50% glycerol, 30% loss of activity after 9 months
-
-20°C, freeze-dried, several months
-
-20°C, purified recombinant protein, stable at pH 6.0 over a period of 1 month
-20°C, several months
-
-60°C, 0.02 mM NADPH, 3 months, frequent freezing and thawing, stable
-
-65°C, frozen tissue, 4 months
-
-70°C, 10% glycerol, less than 5% loss of activity after 9 months
-
-80°C, 1 year
-
4°C for several weeks
-
4°C, 0.1 M imidazole, pH 7.2, 25% glycerol, stable
-
4°C, 40-50% loss of activity after 1 month
-
4°C, a few days
-
4°C, above 1 mg/ml protein concentration, 1 month
-
4°C, pH 7, 20% glycerol, 1 mM EDTA, 1 mM dithiothreitol, at least 90% of activity retained, 2-3 weeks
-
4°C, pure, 20% glycerol, several weeks
-
4°C, several weeks
-
5°C, 50 mM Tris-HCl, pH 8.3, 1 mM EDTA, 1 mM dithiothreitol, 1 M KCl, 0.6 mM 7,8-dihydrofolate, 50% loss of activity overnight
-
5°C, enzyme after affinity chromatography, half-life 2 days
-
ammonium sulfate, 55% saturation, 0°C, for at least 1 year
-
on ice, loss of 20-60% activity after 2 h
-