1.5.1.40: 8-hydroxy-5-deazaflavin:NADPH oxidoreductase

This is an abbreviated version!
For detailed information about 8-hydroxy-5-deazaflavin:NADPH oxidoreductase, go to the full flat file.

Reaction

Synonyms

5-deazaflavin-NADP+ reductase, 8-hydroxy-5-deazaflavin-dependent NADP+ reductase, 8-OH-5-deazaflavin:NADPH oxidoreductase, 8-OH-5dFl:NADPH oxidoreductase, AF0892, F420-dependent NADP oxidoreductase, F420-dependent NADP reductase, F420-dependent NADP+ oxidoreductase, F420:NADPH oxidoreductase, F420H2:NADP oxidoreductase, F420H2:NADP+ oxidoreductase, Fno, Msm_0049, NADP+:F420 oxidoreductase, Tfu-FNO, Tfu_0970

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.1 With NAD⁺ or NADP⁺ as acceptor

                1.5.1.40 8-hydroxy-5-deazaflavin:NADPH oxidoreductase

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Results	in table
94	AA Sequence
1	Application
5	Cloned(Commentary)
10	Cofactor
2	Crystallization (Commentary)
16	General Information
3	General Stability
23	Inhibitors
65	kcat/KM [mM/s]
9	Ki Value [mM]
106	KM Value [mM]
2	Metals/Ions
16	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
44	Organism
1	Oxidation Stability
21	pH Optimum
4	pH Range
1	pI Value
29	Protein Variants
10	Purification (Commentary)
1	Reaction
36	Reference
6	Source Tissue
9	Specific Activity [micromol/min/mg]
6	Storage Stability
57	Substrates and Products (Substrate)
12	Subunits
47	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
3	Temperature Range [°C]
2	Temperature Stability [°C]
71	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.5.1.40 - 8-hydroxy-5-deazaflavin:NADPH oxidoreductase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-75°C, 2 months, at low concentration (0.066 mg/ml), in the presence of ethylene glycol, 20% (v/v), no loss of activity

Methanococcus vannielii

-

725326

4°C or -20°C, enzyme solution is stable for several weeks

Methanogenium organophilum

P80951

724136

4°C, activity in crude extract is stable for a month

Streptomyces griseus

-

711315

4°C, at low concentration (0.066 mg/ml), in the presence of ethylene glycol, 20% (v/v), 22% of the activity is lost

Methanococcus vannielii

-

725326

4°C, at low concentration (0.066 mg/ml), the purified enzyme loses 84% of its activity in 2 days

Methanococcus vannielii

-

725326

4°C, purified enzyme at a protein concentration of 20 mg/ml is completely stable when stored at 4°C in 50 mM Tricine/KOH pH 8.0 containing 0.2 M KCl

Methanothermobacter thermautotrophicus

D9PVP5

724986