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1.5.1.6: formyltetrahydrofolate dehydrogenase

This is an abbreviated version!
For detailed information about formyltetrahydrofolate dehydrogenase, go to the full flat file.

Word Map on EC 1.5.1.6

Reaction

10-formyltetrahydrofolate
+
NADP+
 +
H2O
=
tetrahydrofolate
 +
CO2
 +
NADPH
 +
H+

Synonyms

10-FDH, 10-formyl tetrahydrofolate:NADP oxidoreductase, 10-formyl-H2PtGlu:NADP oxidoreductase, 10-formyl-H4folate dehydrogenase, 10-formyltetrahydrofolate dehydrogenase, 10-fTHF dehydrogenase, 10-FTHFDH, ALDH1L1, ALDH1L2, dehydrogenase, formyltetrahydrofolate, FBP-CI, FBP-CI proteins, FDH, folate-binding proteins, cytosol I, More, mtFDH, N10-formyltetrahydrofolate dehydrogenase, proteins, folate-binding, cytosol I

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.6 formyltetrahydrofolate dehydrogenase

Cloned

Cloned on EC 1.5.1.6 - formyltetrahydrofolate dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
clone expressed in insect cells has both dehydrogenase and hydrolase activity and exhibits almost identical kinetic parameters to the native rat liver enzyme
-
cloning and sequencing of the cDNA encoding FDH from fetal liver
-
cloning of the cDNA encoding FDH
-
cloning of the cDNA encoding FDH, cells from several tumor cell lines are transfected with FDH cDNA cloned into pcDNA 3.1+ vector using LipofectAMINE, expression of FDH inhibits the proliferation of the cell lines as a result of its enzymatic activity
-
expressed in Escherichia coli
-
expressed in Escherichia coli and Sf9 insect cells
-
expressed in Escherichia coli BL21(DE3) Codon Plus cells
-
expression in Escherichia coli
-
expression in Escherichia coli and Pichia pastoris
expression of D124A mutant of FDH and N-terminal domain mutants in Sf9 insect cells, using a baculovirus expression system
-
expression of FDH and its N-terminal and C-terminal domains in insect cells, using a baculovirus expression system, expression of FDH and of its 310 amino acid residue N-terminal domain in Escherichia coli BL21 using the pRSET vector, full-length enzyme expressed in Escherichia coli is nonsoluble, because of the large size of its monomer and uncorrect folding in prokaryotic cells
-
expression of FDH cDNA, subcloned in the pVL 1393 baculovirus vector, in Sf9 insect cells, using the MaxBac expression system
-
expression of FDH in insect cells using the pVL 1393 baculovirus expression vector
-
expression of H106K mutant of FDH in insect cells SF9, using a baculovirus expression system, and of N-terminal domain mutants in Escherichia coli BL 21
-
FDH is cloned
-
transfection of COS-7 and A-549 cells, and expression with green fluorescent protein fusion construct